Improving the Expression of Human Granulocyte Colony Stimulating Factor in Escherichia coli by Reducing the GC-content and Increasing mRNA Folding Free Energy at 5’-Terminal End

Improving the Expression of Human Granulocyte Colony Stimulating Factor in Escherichia coli by Reducing the GC-content and Increasing mRNA Folding Free Energy at 5’-Terminal End

Purpose: Strategy for improving the production of biopharmaceutical protein continues to develop due to increasing market demand. Human granulocyte colony stimulating factor (hG-CSF) is one of biopharmaceutical proteins that has many applications, and easily produced in Escherichia coli expression s...

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Bibliographic Details
Main Authors: Kartika Sari Dewi, Asrul Muhamad Fuad
Format: Article
Language:English
Published: Tabriz University of Medical Sciences 2020-08-01
Series:Advanced Pharmaceutical Bulletin
Subjects:
gcsf
mrna folding free energy
gc-content
5'-terminal end
synonymous codon substitution
codon optimization
Online Access:https://apb.tbzmed.ac.ir/PDF/apb-10-610.pdf

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