DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts

DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts

Gene delivery is one of the steps necessary for gene therapy and for genetic modification. However, delivering DNA into cells is challenging due to its negative charge that leads to repulsion by the negative cell membrane. In the current research, DNA spheres with a DNA encoding to a certain gene we...

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Main Authors: Irena Reytblat, Anat Lipovsky, Aharon Gedanken
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:Journal of Nanomaterials
Online Access:http://dx.doi.org/10.1155/2016/5178029
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spelling doaj-42782373e0f04d5091cebd88957e73a42020-11-24T23:49:36ZengHindawi LimitedJournal of Nanomaterials1687-41101687-41292016-01-01201610.1155/2016/51780295178029DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in YeastsIrena Reytblat0Anat Lipovsky1Aharon Gedanken2Bar-Ilan Institute for Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, 52900 Ramat Gan, IsraelBar-Ilan Institute for Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, 52900 Ramat Gan, IsraelBar-Ilan Institute for Nanotechnology and Advanced Materials, Department of Chemistry, Bar-Ilan University, 52900 Ramat Gan, IsraelGene delivery is one of the steps necessary for gene therapy and for genetic modification. However, delivering DNA into cells is challenging due to its negative charge that leads to repulsion by the negative cell membrane. In the current research, DNA spheres with a DNA encoding to a certain gene were coated with bioavailable polymers, polyethylene imine (PEI) and polycaprolactone (PCL), in a short, one-step sonochemical reaction. The polymers were used in order to neutralize the negative charge of the DNA. Our study shows that the DNA nanospheres not only managed to penetrate the cell without causing it any damage, but also expressed the desired gene inside it.http://dx.doi.org/10.1155/2016/5178029
collection DOAJ
language English
format Article
sources DOAJ
author Irena Reytblat
Anat Lipovsky
Aharon Gedanken
spellingShingle Irena Reytblat
Anat Lipovsky
Aharon Gedanken
DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
Journal of Nanomaterials
author_facet Irena Reytblat
Anat Lipovsky
Aharon Gedanken
author_sort Irena Reytblat
title DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
title_short DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
title_full DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
title_fullStr DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
title_full_unstemmed DNA Microspheres Coated with Bioavailable Polymer as an Efficient Gene Expression Agent in Yeasts
title_sort dna microspheres coated with bioavailable polymer as an efficient gene expression agent in yeasts
publisher Hindawi Limited
series Journal of Nanomaterials
issn 1687-4110
1687-4129
publishDate 2016-01-01
description Gene delivery is one of the steps necessary for gene therapy and for genetic modification. However, delivering DNA into cells is challenging due to its negative charge that leads to repulsion by the negative cell membrane. In the current research, DNA spheres with a DNA encoding to a certain gene were coated with bioavailable polymers, polyethylene imine (PEI) and polycaprolactone (PCL), in a short, one-step sonochemical reaction. The polymers were used in order to neutralize the negative charge of the DNA. Our study shows that the DNA nanospheres not only managed to penetrate the cell without causing it any damage, but also expressed the desired gene inside it.
url http://dx.doi.org/10.1155/2016/5178029
work_keys_str_mv AT irenareytblat dnamicrospherescoatedwithbioavailablepolymerasanefficientgeneexpressionagentinyeasts
AT anatlipovsky dnamicrospherescoatedwithbioavailablepolymerasanefficientgeneexpressionagentinyeasts
AT aharongedanken dnamicrospherescoatedwithbioavailablepolymerasanefficientgeneexpressionagentinyeasts
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