Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis

Tension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of muscle function and development. Recently developed elastic in v...

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Main Authors:	Arne D Hofemeier, Tamara Limon, Till Moritz Muenker, Bernhard Wallmeyer, Alejandro Jurado, Mohammad Ebrahim Afshar, Majid Ebrahimi, Roman Tsukanov, Nazar Oleksiievets, Jörg Enderlein, Penney M Gilbert, Timo Betz
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2021-01-01
Series:	eLife
Subjects:	reconstituted muscle tension sensor muscle development
Online Access:	https://elifesciences.org/articles/60145

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spelling	doaj-426a0733593c4a2d97e1bbcdfd46ad622021-05-05T22:41:42ZengeLife Sciences Publications LtdeLife2050-084X2021-01-011010.7554/eLife.60145Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasisArne D Hofemeier0Tamara Limon1Till Moritz Muenker2Bernhard Wallmeyer3Alejandro Jurado4Mohammad Ebrahim Afshar5Majid Ebrahimi6Roman Tsukanov7Nazar Oleksiievets8Jörg Enderlein9https://orcid.org/0000-0001-5091-7157Penney M Gilbert10https://orcid.org/0000-0001-5509-9616Timo Betz11https://orcid.org/0000-0002-1548-0655Institute for Cell Biology, University of Münster, Münster, GermanyInstitute for Cell Biology, University of Münster, Münster, GermanyInstitute for Cell Biology, University of Münster, Münster, GermanyInstitute for Cell Biology, University of Münster, Münster, GermanyInstitute for Cell Biology, University of Münster, Münster, GermanyInstitute of Biomedical Engineering, University of Toronto, Toronto, Canada; Donnelly Centre, University of Toronto, Toronto, CanadaInstitute of Biomedical Engineering, University of Toronto, Toronto, Canada; Donnelly Centre, University of Toronto, Toronto, Canada3rd Institute of Physics-Biophysics, University of Göttingen, Göttingen, Germany3rd Institute of Physics-Biophysics, University of Göttingen, Göttingen, Germany3rd Institute of Physics-Biophysics, University of Göttingen, Göttingen, Germany; Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen, Göttingen, GermanyInstitute of Biomedical Engineering, University of Toronto, Toronto, Canada; Donnelly Centre, University of Toronto, Toronto, Canada; Department of Cell and Systems Biology, University of Toronto, Toronto, CanadaInstitute for Cell Biology, University of Münster, Münster, Germany; 3rd Institute of Physics-Biophysics, University of Göttingen, Göttingen, Germany; Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Göttingen, Göttingen, GermanyTension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of muscle function and development. Recently developed elastic in vitro culture chambers allow for raising 3D muscle tissue under controlled conditions and to measure global tissue force generation. However, these chambers are inherently incompatible with high-resolution microscopy limiting their usability to global force measurements, and preventing the exploitation of modern fluorescence based investigation methods for live and dynamic measurements. Here, we present a new chamber design pairing global force measurements, quantified from post-deflection, with local tension measurements obtained from elastic hydrogel beads embedded in muscle tissue. High-resolution 3D video microscopy of engineered muscle formation, enabled by the new chamber, shows an early mechanical tissue homeostasis that remains stable in spite of continued myotube maturation.https://elifesciences.org/articles/60145reconstituted muscletension sensormuscle development
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Arne D Hofemeier Tamara Limon Till Moritz Muenker Bernhard Wallmeyer Alejandro Jurado Mohammad Ebrahim Afshar Majid Ebrahimi Roman Tsukanov Nazar Oleksiievets Jörg Enderlein Penney M Gilbert Timo Betz
spellingShingle	Arne D Hofemeier Tamara Limon Till Moritz Muenker Bernhard Wallmeyer Alejandro Jurado Mohammad Ebrahim Afshar Majid Ebrahimi Roman Tsukanov Nazar Oleksiievets Jörg Enderlein Penney M Gilbert Timo Betz Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis eLife reconstituted muscle tension sensor muscle development
author_facet	Arne D Hofemeier Tamara Limon Till Moritz Muenker Bernhard Wallmeyer Alejandro Jurado Mohammad Ebrahim Afshar Majid Ebrahimi Roman Tsukanov Nazar Oleksiievets Jörg Enderlein Penney M Gilbert Timo Betz
author_sort	Arne D Hofemeier
title	Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
title_short	Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
title_full	Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
title_fullStr	Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
title_full_unstemmed	Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
title_sort	global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis
publisher	eLife Sciences Publications Ltd
series	eLife
issn	2050-084X
publishDate	2021-01-01
description	Tension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of muscle function and development. Recently developed elastic in vitro culture chambers allow for raising 3D muscle tissue under controlled conditions and to measure global tissue force generation. However, these chambers are inherently incompatible with high-resolution microscopy limiting their usability to global force measurements, and preventing the exploitation of modern fluorescence based investigation methods for live and dynamic measurements. Here, we present a new chamber design pairing global force measurements, quantified from post-deflection, with local tension measurements obtained from elastic hydrogel beads embedded in muscle tissue. High-resolution 3D video microscopy of engineered muscle formation, enabled by the new chamber, shows an early mechanical tissue homeostasis that remains stable in spite of continued myotube maturation.
topic	reconstituted muscle tension sensor muscle development
url	https://elifesciences.org/articles/60145
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Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis

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