A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement

Purpose. The objective was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with invasive ductal carcinoma of the breast (IDC) with and without lymph node involvement. Methods. Three pooled saliva specimens from women were analyzed. One pooled specimen was from...

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Main Authors: Charles F. Streckfus, Karen A. Storthz, Lenora Bigler, William P. Dubinsky
Format: Article
Language:English
Published: Hindawi Limited 2009-01-01
Series:Journal of Oncology
Online Access:http://dx.doi.org/10.1155/2009/737619
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spelling doaj-42606758e2054c48b0c30d8f7c74c1c02020-11-24T21:57:25ZengHindawi LimitedJournal of Oncology1687-84501687-84692009-01-01200910.1155/2009/737619737619A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node InvolvementCharles F. Streckfus0Karen A. Storthz1Lenora Bigler2William P. Dubinsky3UTHSC—Dental Branch, CCTS—Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USAUTHSC—Dental Branch, CCTS—Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USAUTHSC—Dental Branch, CCTS—Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USAUTHSC—Dental Branch, CCTS—Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USAPurpose. The objective was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with invasive ductal carcinoma of the breast (IDC) with and without lymph node involvement. Methods. Three pooled saliva specimens from women were analyzed. One pooled specimen was from healthy women; another was from women diagnosed with Stage IIa IDC and a specimen from women diagnosed with Stage IIb. The pooled samples were trypsinized and the peptide digests labeled with the appropriate iTRAQ reagent. Labeled peptides from each of the digests were combined and analyzed by reverse phase capillary chromatography on an LC-MS/MS mass spectrometer. Results. The results yielded approximately 174 differentially expressed proteins in the saliva specimens. There were 55 proteins that were common to both cancer stages in comparison to each other and healthy controls while there were 20 proteins unique to Stage IIa and 28 proteins that were unique to Stage IIb.http://dx.doi.org/10.1155/2009/737619
collection DOAJ
language English
format Article
sources DOAJ
author Charles F. Streckfus
Karen A. Storthz
Lenora Bigler
William P. Dubinsky
spellingShingle Charles F. Streckfus
Karen A. Storthz
Lenora Bigler
William P. Dubinsky
A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement
Journal of Oncology
author_facet Charles F. Streckfus
Karen A. Storthz
Lenora Bigler
William P. Dubinsky
author_sort Charles F. Streckfus
title A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement
title_short A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement
title_full A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement
title_fullStr A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement
title_full_unstemmed A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement
title_sort comparison of the proteomic expression in pooled saliva specimens from individuals diagnosed with ductal carcinoma of the breast with and without lymph node involvement
publisher Hindawi Limited
series Journal of Oncology
issn 1687-8450
1687-8469
publishDate 2009-01-01
description Purpose. The objective was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with invasive ductal carcinoma of the breast (IDC) with and without lymph node involvement. Methods. Three pooled saliva specimens from women were analyzed. One pooled specimen was from healthy women; another was from women diagnosed with Stage IIa IDC and a specimen from women diagnosed with Stage IIb. The pooled samples were trypsinized and the peptide digests labeled with the appropriate iTRAQ reagent. Labeled peptides from each of the digests were combined and analyzed by reverse phase capillary chromatography on an LC-MS/MS mass spectrometer. Results. The results yielded approximately 174 differentially expressed proteins in the saliva specimens. There were 55 proteins that were common to both cancer stages in comparison to each other and healthy controls while there were 20 proteins unique to Stage IIa and 28 proteins that were unique to Stage IIb.
url http://dx.doi.org/10.1155/2009/737619
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