A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement
Purpose. The objective was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with invasive ductal carcinoma of the breast (IDC) with and without lymph node involvement. Methods. Three pooled saliva specimens from women were analyzed. One pooled specimen was from...
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2009-01-01
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Series: | Journal of Oncology |
Online Access: | http://dx.doi.org/10.1155/2009/737619 |
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doaj-42606758e2054c48b0c30d8f7c74c1c02020-11-24T21:57:25ZengHindawi LimitedJournal of Oncology1687-84501687-84692009-01-01200910.1155/2009/737619737619A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node InvolvementCharles F. Streckfus0Karen A. Storthz1Lenora Bigler2William P. Dubinsky3UTHSC—Dental Branch, CCTS—Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USAUTHSC—Dental Branch, CCTS—Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USAUTHSC—Dental Branch, CCTS—Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USAUTHSC—Dental Branch, CCTS—Salivary Proteomics Core, 6516 M.D. Anderson Blvd., Room 4.133f, Houston, TX 77030, USAPurpose. The objective was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with invasive ductal carcinoma of the breast (IDC) with and without lymph node involvement. Methods. Three pooled saliva specimens from women were analyzed. One pooled specimen was from healthy women; another was from women diagnosed with Stage IIa IDC and a specimen from women diagnosed with Stage IIb. The pooled samples were trypsinized and the peptide digests labeled with the appropriate iTRAQ reagent. Labeled peptides from each of the digests were combined and analyzed by reverse phase capillary chromatography on an LC-MS/MS mass spectrometer. Results. The results yielded approximately 174 differentially expressed proteins in the saliva specimens. There were 55 proteins that were common to both cancer stages in comparison to each other and healthy controls while there were 20 proteins unique to Stage IIa and 28 proteins that were unique to Stage IIb.http://dx.doi.org/10.1155/2009/737619 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Charles F. Streckfus Karen A. Storthz Lenora Bigler William P. Dubinsky |
spellingShingle |
Charles F. Streckfus Karen A. Storthz Lenora Bigler William P. Dubinsky A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement Journal of Oncology |
author_facet |
Charles F. Streckfus Karen A. Storthz Lenora Bigler William P. Dubinsky |
author_sort |
Charles F. Streckfus |
title |
A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement |
title_short |
A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement |
title_full |
A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement |
title_fullStr |
A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement |
title_full_unstemmed |
A Comparison of the Proteomic Expression in Pooled Saliva Specimens from Individuals Diagnosed with Ductal Carcinoma of the Breast with and without Lymph Node Involvement |
title_sort |
comparison of the proteomic expression in pooled saliva specimens from individuals diagnosed with ductal carcinoma of the breast with and without lymph node involvement |
publisher |
Hindawi Limited |
series |
Journal of Oncology |
issn |
1687-8450 1687-8469 |
publishDate |
2009-01-01 |
description |
Purpose. The objective was to compare the salivary protein profiles of saliva specimens from individuals diagnosed with invasive ductal carcinoma of the breast (IDC) with and without lymph node involvement. Methods. Three pooled saliva specimens from women were analyzed. One pooled specimen was from healthy women; another was from women diagnosed with Stage IIa IDC and a specimen from women diagnosed with Stage IIb. The pooled samples were trypsinized and the peptide digests labeled with the appropriate iTRAQ reagent. Labeled peptides from each of the digests were combined and analyzed by reverse phase capillary chromatography on an LC-MS/MS mass spectrometer. Results. The results yielded approximately 174 differentially expressed proteins in the saliva specimens. There were 55 proteins that were common to both cancer stages in comparison to each other and healthy controls while there were 20 proteins unique to Stage IIa and 28 proteins that were unique to Stage IIb. |
url |
http://dx.doi.org/10.1155/2009/737619 |
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