Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology
Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in...
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00213/full |
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doaj-42571fb9b6a24ed0a05add8531bc1d992020-11-25T02:50:26ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-03-01810.3389/fbioe.2020.00213526426Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic BiologyNadanai Laohakunakorn0Laura Grasemann1Barbora Lavickova2Grégoire Michielin3Amir Shahein4Zoe Swank5Sebastian J. Maerkl6School of Biological Sciences, Institute of Quantitative Biology, Biochemistry, and Biotechnology, University of Edinburgh, Edinburgh, United KingdomSchool of Engineering, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandSchool of Engineering, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandSchool of Engineering, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandSchool of Engineering, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandSchool of Engineering, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandSchool of Engineering, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandCell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated increased throughput, compartmentalization, and spatial control of cell-free protein synthesis reactions. Combined with a deeper understanding of the cell-free systems themselves, these advances improve our ability to address a range of scientific questions. By mastering control of the cell-free platform, we will be in a position to construct increasingly complex biomolecular systems, and approach natural biological complexity in a bottom-up manner.https://www.frontiersin.org/article/10.3389/fbioe.2020.00213/fullcell-free synthetic biologycell-free protein synthesisin vitro reconstitutionmicrofluidicscompartmentalizationartificial cell |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Nadanai Laohakunakorn Laura Grasemann Barbora Lavickova Grégoire Michielin Amir Shahein Zoe Swank Sebastian J. Maerkl |
| spellingShingle |
Nadanai Laohakunakorn Laura Grasemann Barbora Lavickova Grégoire Michielin Amir Shahein Zoe Swank Sebastian J. Maerkl Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology Frontiers in Bioengineering and Biotechnology cell-free synthetic biology cell-free protein synthesis in vitro reconstitution microfluidics compartmentalization artificial cell |
| author_facet |
Nadanai Laohakunakorn Laura Grasemann Barbora Lavickova Grégoire Michielin Amir Shahein Zoe Swank Sebastian J. Maerkl |
| author_sort |
Nadanai Laohakunakorn |
| title |
Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology |
| title_short |
Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology |
| title_full |
Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology |
| title_fullStr |
Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology |
| title_full_unstemmed |
Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology |
| title_sort |
bottom-up construction of complex biomolecular systems with cell-free synthetic biology |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Bioengineering and Biotechnology |
| issn |
2296-4185 |
| publishDate |
2020-03-01 |
| description |
Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated increased throughput, compartmentalization, and spatial control of cell-free protein synthesis reactions. Combined with a deeper understanding of the cell-free systems themselves, these advances improve our ability to address a range of scientific questions. By mastering control of the cell-free platform, we will be in a position to construct increasingly complex biomolecular systems, and approach natural biological complexity in a bottom-up manner. |
| topic |
cell-free synthetic biology cell-free protein synthesis in vitro reconstitution microfluidics compartmentalization artificial cell |
| url |
https://www.frontiersin.org/article/10.3389/fbioe.2020.00213/full |
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