Impact of growth factor content on proliferation of mesenchymal stromal cells derived from adipose tissue.

Autologous adipose tissue (AT) transfer has gained widespread acceptance and is used for a broad variety of regenerative clinical indications. It is assumed that the successful outcome of AT transfer essentially depends on the amount of autocrine-generated growth factors (GF). It is supposed that se...

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Main Authors: Katrin C Franz, Christoph V Suschek, Vera Grotheer, Mehmet Akbas, Norbert Pallua
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0230265
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spelling doaj-423bd16bd0ad44d184065f4da97255952021-03-03T21:37:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01154e023026510.1371/journal.pone.0230265Impact of growth factor content on proliferation of mesenchymal stromal cells derived from adipose tissue.Katrin C FranzChristoph V SuschekVera GrotheerMehmet AkbasNorbert PalluaAutologous adipose tissue (AT) transfer has gained widespread acceptance and is used for a broad variety of regenerative clinical indications. It is assumed that the successful outcome of AT transfer essentially depends on the amount of autocrine-generated growth factors (GF). It is supposed that several GF enhance and improve the anatomic and functional integration of the transplanted AT grafts at the site of implantation. In the present study we have investigated for the first time the correlation between the concentration of GF of freshly isolated AT and the proliferation and migration capacity of mesenchymal stroma cells (MSCs) derived from the respective AT sample. We here show that the proliferation and migration capacity of MSCs strongly depends on the GF content of the AT the cells were isolated from but in an inversely proportional manner. The lower the GF content of an AT sample was, the higher was the proliferation and migration capacity of the respective MSC population contained in the AT and vice versa. Furthermore, we found that supplementation with recombinant GFs only in the case of AT samples with low but not with higher growth factor contents led to a significant enhancement of proliferation and migration of the AT-resident MSCs. As we further show, this inefficiency of GFs to enhance MSC proliferation and migration in AT samples with high GF contents indicates a GF-mediated negative feedback mechanism leading to an impaired GF signaling in MSC obtained from those AT samples. Our results might explain why the successful use of AT grafting is frequently limited by low and unpredictable survival rates, and we suggest to use the knowledge of GF content of harvested AT as a predictive clinical parameter for risk assessment of the therapeutic outcome of autologous AT transfer.https://doi.org/10.1371/journal.pone.0230265
collection DOAJ
language English
format Article
sources DOAJ
author Katrin C Franz
Christoph V Suschek
Vera Grotheer
Mehmet Akbas
Norbert Pallua
spellingShingle Katrin C Franz
Christoph V Suschek
Vera Grotheer
Mehmet Akbas
Norbert Pallua
Impact of growth factor content on proliferation of mesenchymal stromal cells derived from adipose tissue.
PLoS ONE
author_facet Katrin C Franz
Christoph V Suschek
Vera Grotheer
Mehmet Akbas
Norbert Pallua
author_sort Katrin C Franz
title Impact of growth factor content on proliferation of mesenchymal stromal cells derived from adipose tissue.
title_short Impact of growth factor content on proliferation of mesenchymal stromal cells derived from adipose tissue.
title_full Impact of growth factor content on proliferation of mesenchymal stromal cells derived from adipose tissue.
title_fullStr Impact of growth factor content on proliferation of mesenchymal stromal cells derived from adipose tissue.
title_full_unstemmed Impact of growth factor content on proliferation of mesenchymal stromal cells derived from adipose tissue.
title_sort impact of growth factor content on proliferation of mesenchymal stromal cells derived from adipose tissue.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2020-01-01
description Autologous adipose tissue (AT) transfer has gained widespread acceptance and is used for a broad variety of regenerative clinical indications. It is assumed that the successful outcome of AT transfer essentially depends on the amount of autocrine-generated growth factors (GF). It is supposed that several GF enhance and improve the anatomic and functional integration of the transplanted AT grafts at the site of implantation. In the present study we have investigated for the first time the correlation between the concentration of GF of freshly isolated AT and the proliferation and migration capacity of mesenchymal stroma cells (MSCs) derived from the respective AT sample. We here show that the proliferation and migration capacity of MSCs strongly depends on the GF content of the AT the cells were isolated from but in an inversely proportional manner. The lower the GF content of an AT sample was, the higher was the proliferation and migration capacity of the respective MSC population contained in the AT and vice versa. Furthermore, we found that supplementation with recombinant GFs only in the case of AT samples with low but not with higher growth factor contents led to a significant enhancement of proliferation and migration of the AT-resident MSCs. As we further show, this inefficiency of GFs to enhance MSC proliferation and migration in AT samples with high GF contents indicates a GF-mediated negative feedback mechanism leading to an impaired GF signaling in MSC obtained from those AT samples. Our results might explain why the successful use of AT grafting is frequently limited by low and unpredictable survival rates, and we suggest to use the knowledge of GF content of harvested AT as a predictive clinical parameter for risk assessment of the therapeutic outcome of autologous AT transfer.
url https://doi.org/10.1371/journal.pone.0230265
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