In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants
Adhesion to the intestinal epithelium is considered to be one of the selection criteria for probiotics strain. In this study, the adhesion of four different Lactobacillus strains with potential probiotics properties, i.e. L. paracasei MSMC39-1, L. casei MSMC39-3, L. salivarius MSMC105-3 and L. pla...
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doaj-423a7b58a073492b961eef008a80fa5b2020-11-25T01:00:18ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952018-02-01401697410.14456/sjst-psu.2018.14In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infantsKwannan Nantavisai0Srisombat Puttikamonkul1Kruawan Chotelersak2Malai Taweechotipatr3Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Watthana, Bangkok, 10110 ThailandDepartment of Microbiology, Faculty of Medicine, Srinakharinwirot University, Watthana, Bangkok, 10110 ThailandDepartment of Microbiology, Faculty of Medicine, Srinakharinwirot University, Watthana, Bangkok, 10110 ThailandDepartment of Microbiology, Faculty of Medicine, Srinakharinwirot University, Watthana, Bangkok, 10110 ThailandAdhesion to the intestinal epithelium is considered to be one of the selection criteria for probiotics strain. In this study, the adhesion of four different Lactobacillus strains with potential probiotics properties, i.e. L. paracasei MSMC39-1, L. casei MSMC39-3, L. salivarius MSMC105-3 and L. plantarum MSMC171-1, was studied using Caco-2 cell line as an in vitro model for intestinal epithelium. Among four different Lactobacillus strains, L. salivarius MSMC105-3 was the most adhesive strain showing about 3.5 percent of adhesion index. Thus, this strain was selected to examine for its ability to inhibit the adhesion of pathogenic Salmonella Typhi DMST5784 and Shigella dysenteriae DMST15111 to Caco-2 cells. The results showed that L. salivarius MSMC105-3 whole cell and its cell-free culture supernatant could inhibit the adhesion of pathogens. The results from this study indicated that both L. salivarius MSMC105-3 itself and its substances secreted into culture supernatant had the ability to reduce the adhesion of enteropathogens to Caco-2 cells.http://rdo.psu.ac.th/sjstweb/journal/40-1/40-1-8.pdfprobioticsLactobacillusCaco-2 celladhesioncompetitionSalmonella TyphiShigella dysenteriae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kwannan Nantavisai Srisombat Puttikamonkul Kruawan Chotelersak Malai Taweechotipatr |
spellingShingle |
Kwannan Nantavisai Srisombat Puttikamonkul Kruawan Chotelersak Malai Taweechotipatr In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants Songklanakarin Journal of Science and Technology (SJST) probiotics Lactobacillus Caco-2 cell adhesion competition Salmonella Typhi Shigella dysenteriae |
author_facet |
Kwannan Nantavisai Srisombat Puttikamonkul Kruawan Chotelersak Malai Taweechotipatr |
author_sort |
Kwannan Nantavisai |
title |
In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants |
title_short |
In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants |
title_full |
In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants |
title_fullStr |
In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants |
title_full_unstemmed |
In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants |
title_sort |
in vitro adhesion property and competition against enteropathogens of lactobacillus strains isolated from thai infants |
publisher |
Prince of Songkla University |
series |
Songklanakarin Journal of Science and Technology (SJST) |
issn |
0125-3395 |
publishDate |
2018-02-01 |
description |
Adhesion to the intestinal epithelium is considered to be one of the selection criteria for probiotics strain. In this study, the
adhesion of four different Lactobacillus strains with potential probiotics properties, i.e. L. paracasei MSMC39-1, L. casei
MSMC39-3, L. salivarius MSMC105-3 and L. plantarum MSMC171-1, was studied using Caco-2 cell line as an in vitro model
for intestinal epithelium. Among four different Lactobacillus strains, L. salivarius MSMC105-3 was the most adhesive strain
showing about 3.5 percent of adhesion index. Thus, this strain was selected to examine for its ability to inhibit the adhesion of
pathogenic Salmonella Typhi DMST5784 and Shigella dysenteriae DMST15111 to Caco-2 cells. The results showed that L.
salivarius MSMC105-3 whole cell and its cell-free culture supernatant could inhibit the adhesion of pathogens. The results from
this study indicated that both L. salivarius MSMC105-3 itself and its substances secreted into culture supernatant had the ability
to reduce the adhesion of enteropathogens to Caco-2 cells. |
topic |
probiotics Lactobacillus Caco-2 cell adhesion competition Salmonella Typhi Shigella dysenteriae |
url |
http://rdo.psu.ac.th/sjstweb/journal/40-1/40-1-8.pdf |
work_keys_str_mv |
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1725214144608600064 |