In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants

Adhesion to the intestinal epithelium is considered to be one of the selection criteria for probiotics strain. In this study, the adhesion of four different Lactobacillus strains with potential probiotics properties, i.e. L. paracasei MSMC39-1, L. casei MSMC39-3, L. salivarius MSMC105-3 and L. pla...

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Main Authors: Kwannan Nantavisai, Srisombat Puttikamonkul, Kruawan Chotelersak, Malai Taweechotipatr
Format: Article
Language:English
Published: Prince of Songkla University 2018-02-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
Online Access:http://rdo.psu.ac.th/sjstweb/journal/40-1/40-1-8.pdf
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spelling doaj-423a7b58a073492b961eef008a80fa5b2020-11-25T01:00:18ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952018-02-01401697410.14456/sjst-psu.2018.14In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infantsKwannan Nantavisai0Srisombat Puttikamonkul1Kruawan Chotelersak2Malai Taweechotipatr3Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Watthana, Bangkok, 10110 ThailandDepartment of Microbiology, Faculty of Medicine, Srinakharinwirot University, Watthana, Bangkok, 10110 ThailandDepartment of Microbiology, Faculty of Medicine, Srinakharinwirot University, Watthana, Bangkok, 10110 ThailandDepartment of Microbiology, Faculty of Medicine, Srinakharinwirot University, Watthana, Bangkok, 10110 ThailandAdhesion to the intestinal epithelium is considered to be one of the selection criteria for probiotics strain. In this study, the adhesion of four different Lactobacillus strains with potential probiotics properties, i.e. L. paracasei MSMC39-1, L. casei MSMC39-3, L. salivarius MSMC105-3 and L. plantarum MSMC171-1, was studied using Caco-2 cell line as an in vitro model for intestinal epithelium. Among four different Lactobacillus strains, L. salivarius MSMC105-3 was the most adhesive strain showing about 3.5 percent of adhesion index. Thus, this strain was selected to examine for its ability to inhibit the adhesion of pathogenic Salmonella Typhi DMST5784 and Shigella dysenteriae DMST15111 to Caco-2 cells. The results showed that L. salivarius MSMC105-3 whole cell and its cell-free culture supernatant could inhibit the adhesion of pathogens. The results from this study indicated that both L. salivarius MSMC105-3 itself and its substances secreted into culture supernatant had the ability to reduce the adhesion of enteropathogens to Caco-2 cells.http://rdo.psu.ac.th/sjstweb/journal/40-1/40-1-8.pdfprobioticsLactobacillusCaco-2 celladhesioncompetitionSalmonella TyphiShigella dysenteriae
collection DOAJ
language English
format Article
sources DOAJ
author Kwannan Nantavisai
Srisombat Puttikamonkul
Kruawan Chotelersak
Malai Taweechotipatr
spellingShingle Kwannan Nantavisai
Srisombat Puttikamonkul
Kruawan Chotelersak
Malai Taweechotipatr
In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants
Songklanakarin Journal of Science and Technology (SJST)
probiotics
Lactobacillus
Caco-2 cell
adhesion
competition
Salmonella Typhi
Shigella dysenteriae
author_facet Kwannan Nantavisai
Srisombat Puttikamonkul
Kruawan Chotelersak
Malai Taweechotipatr
author_sort Kwannan Nantavisai
title In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants
title_short In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants
title_full In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants
title_fullStr In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants
title_full_unstemmed In vitro adhesion property and competition against enteropathogens of Lactobacillus strains isolated from Thai infants
title_sort in vitro adhesion property and competition against enteropathogens of lactobacillus strains isolated from thai infants
publisher Prince of Songkla University
series Songklanakarin Journal of Science and Technology (SJST)
issn 0125-3395
publishDate 2018-02-01
description Adhesion to the intestinal epithelium is considered to be one of the selection criteria for probiotics strain. In this study, the adhesion of four different Lactobacillus strains with potential probiotics properties, i.e. L. paracasei MSMC39-1, L. casei MSMC39-3, L. salivarius MSMC105-3 and L. plantarum MSMC171-1, was studied using Caco-2 cell line as an in vitro model for intestinal epithelium. Among four different Lactobacillus strains, L. salivarius MSMC105-3 was the most adhesive strain showing about 3.5 percent of adhesion index. Thus, this strain was selected to examine for its ability to inhibit the adhesion of pathogenic Salmonella Typhi DMST5784 and Shigella dysenteriae DMST15111 to Caco-2 cells. The results showed that L. salivarius MSMC105-3 whole cell and its cell-free culture supernatant could inhibit the adhesion of pathogens. The results from this study indicated that both L. salivarius MSMC105-3 itself and its substances secreted into culture supernatant had the ability to reduce the adhesion of enteropathogens to Caco-2 cells.
topic probiotics
Lactobacillus
Caco-2 cell
adhesion
competition
Salmonella Typhi
Shigella dysenteriae
url http://rdo.psu.ac.th/sjstweb/journal/40-1/40-1-8.pdf
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