Chitinolytic bactery activity isolated from the mud fields

A chitinolytic microorganism had been isolated from mud fields in Ketintang Surabaya area by using a minimal media containing 0.4% colloidal chitin. Activity assay is based on released N-acetyl-glucosamine which reacted with 3,5 dinitrosalicyclic acid. Mud fields produce 63 bacterial isolates with c...

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Main Authors:	Nuniek Herdyastuti, Sari Edi Cahyaningrum, Tri Joko Raharjo, Mudasir Mudasir, Sabirin Matsjeh
Format:	Article
Language:	English
Published:	Indonesian Biological Society 2012-01-01
Series:	Journal of Biological Researches
Subjects:	Chitin Coloidal chitin Chitinolytic microorganism Mud fields
Online Access:	http://berkalahayati.org/journal/f61408e3

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spelling	doaj-422c6352889b443289d8ec25556a57cb2020-11-24T23:31:36ZengIndonesian Biological SocietyJournal of Biological Researches0852-68342337-389X2012-01-0115210711110.23869/bphjbr.15.2.20102Chitinolytic bactery activity isolated from the mud fieldsNuniek Herdyastuti0Sari Edi Cahyaningrum1Tri Joko Raharjo2Mudasir Mudasir3Sabirin Matsjeh4Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231A chitinolytic microorganism had been isolated from mud fields in Ketintang Surabaya area by using a minimal media containing 0.4% colloidal chitin. Activity assay is based on released N-acetyl-glucosamine which reacted with 3,5 dinitrosalicyclic acid. Mud fields produce 63 bacterial isolates with chitinase activity (TNH1 – TNH63 isolates). The highest activity was shown by TNH11 isolate with specific activity 1.27 U/mg. TNH11 isolates was Gram negative, rod-cocoid cell, has a colony of yellow, round shape, convex elevation, flat margin and the colony diameter 3–5 mm.http://berkalahayati.org/journal/f61408e3ChitinColoidal chitinChitinolytic microorganismMud fields
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Nuniek Herdyastuti Sari Edi Cahyaningrum Tri Joko Raharjo Mudasir Mudasir Sabirin Matsjeh
spellingShingle	Nuniek Herdyastuti Sari Edi Cahyaningrum Tri Joko Raharjo Mudasir Mudasir Sabirin Matsjeh Chitinolytic bactery activity isolated from the mud fields Journal of Biological Researches Chitin Coloidal chitin Chitinolytic microorganism Mud fields
author_facet	Nuniek Herdyastuti Sari Edi Cahyaningrum Tri Joko Raharjo Mudasir Mudasir Sabirin Matsjeh
author_sort	Nuniek Herdyastuti
title	Chitinolytic bactery activity isolated from the mud fields
title_short	Chitinolytic bactery activity isolated from the mud fields
title_full	Chitinolytic bactery activity isolated from the mud fields
title_fullStr	Chitinolytic bactery activity isolated from the mud fields
title_full_unstemmed	Chitinolytic bactery activity isolated from the mud fields
title_sort	chitinolytic bactery activity isolated from the mud fields
publisher	Indonesian Biological Society
series	Journal of Biological Researches
issn	0852-6834 2337-389X
publishDate	2012-01-01
description	A chitinolytic microorganism had been isolated from mud fields in Ketintang Surabaya area by using a minimal media containing 0.4% colloidal chitin. Activity assay is based on released N-acetyl-glucosamine which reacted with 3,5 dinitrosalicyclic acid. Mud fields produce 63 bacterial isolates with chitinase activity (TNH1 – TNH63 isolates). The highest activity was shown by TNH11 isolate with specific activity 1.27 U/mg. TNH11 isolates was Gram negative, rod-cocoid cell, has a colony of yellow, round shape, convex elevation, flat margin and the colony diameter 3–5 mm.
topic	Chitin Coloidal chitin Chitinolytic microorganism Mud fields
url	http://berkalahayati.org/journal/f61408e3
work_keys_str_mv	AT nuniekherdyastuti chitinolyticbacteryactivityisolatedfromthemudfields AT sariedicahyaningrum chitinolyticbacteryactivityisolatedfromthemudfields AT trijokoraharjo chitinolyticbacteryactivityisolatedfromthemudfields AT mudasirmudasir chitinolyticbacteryactivityisolatedfromthemudfields AT sabirinmatsjeh chitinolyticbacteryactivityisolatedfromthemudfields
_version_	1725537079848337408

Chitinolytic bactery activity isolated from the mud fields

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