EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS

The aim of the work was to investigate the effect of the intracranial catheter placement on the metabolic profile of rat microglia. Microglial cells were isolated by the centrifugation in Percoll gradient. Oxidative metabolism and phagocytic activity were investigated by flow cytometry. Arginase act...

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Main Authors: Y. Hurmach, М. Rudyk, V. Svyatetskaya, L. Skivka
Format: Article
Language:English
Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2018-08-01
Series:Biotechnologia Acta
Subjects:
Online Access:http://biotechnology.kiev.ua/images/storage/4_2018/hurmach_4_2018.pdf
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spelling doaj-4217f7c3db424424bd1d9c557d1e75272020-11-24T21:13:40ZengNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.Biotechnologia Acta2410-77512410-776X2018-08-01114849010.15407/biotechEFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATSY. Hurmach0М. Rudyk1V. Svyatetskaya2L. Skivka3Taras Shevchenko National University of Kyiv,, ESC “Institute of Biology and Medicine” Kyiv, UkraineTaras Shevchenko National University of Kyiv,, ESC “Institute of Biology and Medicine” Kyiv, UkraineTaras Shevchenko National University of Kyiv,, ESC “Institute of Biology and Medicine” Kyiv, UkraineTaras Shevchenko National University of Kyiv,, ESC “Institute of Biology and Medicine” Kyiv, UkraineThe aim of the work was to investigate the effect of the intracranial catheter placement on the metabolic profile of rat microglia. Microglial cells were isolated by the centrifugation in Percoll gradient. Oxidative metabolism and phagocytic activity were investigated by flow cytometry. Arginase activity was examined by colorimetric method. Nitrite level was assayed in Griess reaction. It was found that intracranial catheter placement caused down-regulation of nitrite synthesis by 3 times, augmentation of the reactive oxygen species generation by 1.5 times, and slightly decreased microglia phagocytic activity. Thus, intracranial catheter placement causes long term anti-inflammatory shift of microglia metabolism in rats. http://biotechnology.kiev.ua/images/storage/4_2018/hurmach_4_2018.pdfphagocytesmicrogliametabolic profileintracranial catheter.
collection DOAJ
language English
format Article
sources DOAJ
author Y. Hurmach
М. Rudyk
V. Svyatetskaya
L. Skivka
spellingShingle Y. Hurmach
М. Rudyk
V. Svyatetskaya
L. Skivka
EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS
Biotechnologia Acta
phagocytes
microglia
metabolic profile
intracranial catheter.
author_facet Y. Hurmach
М. Rudyk
V. Svyatetskaya
L. Skivka
author_sort Y. Hurmach
title EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS
title_short EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS
title_full EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS
title_fullStr EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS
title_full_unstemmed EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS
title_sort effect of intracranial catheter placement on microglia metabolic profile of rats
publisher National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
series Biotechnologia Acta
issn 2410-7751
2410-776X
publishDate 2018-08-01
description The aim of the work was to investigate the effect of the intracranial catheter placement on the metabolic profile of rat microglia. Microglial cells were isolated by the centrifugation in Percoll gradient. Oxidative metabolism and phagocytic activity were investigated by flow cytometry. Arginase activity was examined by colorimetric method. Nitrite level was assayed in Griess reaction. It was found that intracranial catheter placement caused down-regulation of nitrite synthesis by 3 times, augmentation of the reactive oxygen species generation by 1.5 times, and slightly decreased microglia phagocytic activity. Thus, intracranial catheter placement causes long term anti-inflammatory shift of microglia metabolism in rats.
topic phagocytes
microglia
metabolic profile
intracranial catheter.
url http://biotechnology.kiev.ua/images/storage/4_2018/hurmach_4_2018.pdf
work_keys_str_mv AT yhurmach effectofintracranialcatheterplacementonmicrogliametabolicprofileofrats
AT mrudyk effectofintracranialcatheterplacementonmicrogliametabolicprofileofrats
AT vsvyatetskaya effectofintracranialcatheterplacementonmicrogliametabolicprofileofrats
AT lskivka effectofintracranialcatheterplacementonmicrogliametabolicprofileofrats
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