EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS
The aim of the work was to investigate the effect of the intracranial catheter placement on the metabolic profile of rat microglia. Microglial cells were isolated by the centrifugation in Percoll gradient. Oxidative metabolism and phagocytic activity were investigated by flow cytometry. Arginase act...
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National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2018-08-01
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Online Access: | http://biotechnology.kiev.ua/images/storage/4_2018/hurmach_4_2018.pdf |
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doaj-4217f7c3db424424bd1d9c557d1e75272020-11-24T21:13:40ZengNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.Biotechnologia Acta2410-77512410-776X2018-08-01114849010.15407/biotechEFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATSY. Hurmach0М. Rudyk1V. Svyatetskaya2L. Skivka3Taras Shevchenko National University of Kyiv,, ESC “Institute of Biology and Medicine” Kyiv, UkraineTaras Shevchenko National University of Kyiv,, ESC “Institute of Biology and Medicine” Kyiv, UkraineTaras Shevchenko National University of Kyiv,, ESC “Institute of Biology and Medicine” Kyiv, UkraineTaras Shevchenko National University of Kyiv,, ESC “Institute of Biology and Medicine” Kyiv, UkraineThe aim of the work was to investigate the effect of the intracranial catheter placement on the metabolic profile of rat microglia. Microglial cells were isolated by the centrifugation in Percoll gradient. Oxidative metabolism and phagocytic activity were investigated by flow cytometry. Arginase activity was examined by colorimetric method. Nitrite level was assayed in Griess reaction. It was found that intracranial catheter placement caused down-regulation of nitrite synthesis by 3 times, augmentation of the reactive oxygen species generation by 1.5 times, and slightly decreased microglia phagocytic activity. Thus, intracranial catheter placement causes long term anti-inflammatory shift of microglia metabolism in rats. http://biotechnology.kiev.ua/images/storage/4_2018/hurmach_4_2018.pdfphagocytesmicrogliametabolic profileintracranial catheter. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Y. Hurmach М. Rudyk V. Svyatetskaya L. Skivka |
spellingShingle |
Y. Hurmach М. Rudyk V. Svyatetskaya L. Skivka EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS Biotechnologia Acta phagocytes microglia metabolic profile intracranial catheter. |
author_facet |
Y. Hurmach М. Rudyk V. Svyatetskaya L. Skivka |
author_sort |
Y. Hurmach |
title |
EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS |
title_short |
EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS |
title_full |
EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS |
title_fullStr |
EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS |
title_full_unstemmed |
EFFECT OF INTRACRANIAL CATHETER PLACEMENT ON MICROGLIA METABOLIC PROFILE OF RATS |
title_sort |
effect of intracranial catheter placement on microglia metabolic profile of rats |
publisher |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. |
series |
Biotechnologia Acta |
issn |
2410-7751 2410-776X |
publishDate |
2018-08-01 |
description |
The aim of the work was to investigate the effect of the intracranial catheter placement on the metabolic profile of rat microglia. Microglial cells were isolated by the centrifugation in Percoll gradient. Oxidative metabolism and phagocytic activity were investigated by flow cytometry. Arginase activity was examined by colorimetric method. Nitrite level was assayed in Griess reaction. It was found that intracranial catheter placement caused down-regulation of nitrite synthesis by 3 times, augmentation of the reactive oxygen species generation by 1.5 times, and slightly decreased microglia phagocytic activity. Thus, intracranial catheter placement causes long term anti-inflammatory shift of microglia metabolism in rats. |
topic |
phagocytes microglia metabolic profile intracranial catheter. |
url |
http://biotechnology.kiev.ua/images/storage/4_2018/hurmach_4_2018.pdf |
work_keys_str_mv |
AT yhurmach effectofintracranialcatheterplacementonmicrogliametabolicprofileofrats AT mrudyk effectofintracranialcatheterplacementonmicrogliametabolicprofileofrats AT vsvyatetskaya effectofintracranialcatheterplacementonmicrogliametabolicprofileofrats AT lskivka effectofintracranialcatheterplacementonmicrogliametabolicprofileofrats |
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1716748395713921024 |