A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules
Bacterial allosteric transcription factors can sense and respond to a variety of small molecules. Here the authors present CaT-SMelor which uses Cas12a and allosteric transcription factors to detect small molecules in the nanomolar range.
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2019-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-11648-1 |
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doaj-4207fa0937a94606979b5c111fae58fa |
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| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Mindong Liang Zilong Li Weishan Wang Jiakun Liu Leshi Liu Guoliang Zhu Loganathan Karthik Man Wang Ke-Feng Wang Zhong Wang Jing Yu Yuting Shuai Jiaming Yu Lu Zhang Zhiheng Yang Chuan Li Qian Zhang Tong Shi Liming Zhou Feng Xie Huanqin Dai Xueting Liu Jingyu Zhang Guang Liu Ying Zhuo Buchang Zhang Chenli Liu Shanshan Li Xuekui Xia Yaojun Tong Yanwen Liu Gil Alterovitz Gao-Yi Tan Li-Xin Zhang |
| spellingShingle |
Mindong Liang Zilong Li Weishan Wang Jiakun Liu Leshi Liu Guoliang Zhu Loganathan Karthik Man Wang Ke-Feng Wang Zhong Wang Jing Yu Yuting Shuai Jiaming Yu Lu Zhang Zhiheng Yang Chuan Li Qian Zhang Tong Shi Liming Zhou Feng Xie Huanqin Dai Xueting Liu Jingyu Zhang Guang Liu Ying Zhuo Buchang Zhang Chenli Liu Shanshan Li Xuekui Xia Yaojun Tong Yanwen Liu Gil Alterovitz Gao-Yi Tan Li-Xin Zhang A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules Nature Communications |
| author_facet |
Mindong Liang Zilong Li Weishan Wang Jiakun Liu Leshi Liu Guoliang Zhu Loganathan Karthik Man Wang Ke-Feng Wang Zhong Wang Jing Yu Yuting Shuai Jiaming Yu Lu Zhang Zhiheng Yang Chuan Li Qian Zhang Tong Shi Liming Zhou Feng Xie Huanqin Dai Xueting Liu Jingyu Zhang Guang Liu Ying Zhuo Buchang Zhang Chenli Liu Shanshan Li Xuekui Xia Yaojun Tong Yanwen Liu Gil Alterovitz Gao-Yi Tan Li-Xin Zhang |
| author_sort |
Mindong Liang |
| title |
A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules |
| title_short |
A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules |
| title_full |
A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules |
| title_fullStr |
A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules |
| title_full_unstemmed |
A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules |
| title_sort |
crispr-cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2019-08-01 |
| description |
Bacterial allosteric transcription factors can sense and respond to a variety of small molecules. Here the authors present CaT-SMelor which uses Cas12a and allosteric transcription factors to detect small molecules in the nanomolar range. |
| url |
https://doi.org/10.1038/s41467-019-11648-1 |
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doaj-4207fa0937a94606979b5c111fae58fa2021-05-11T12:05:01ZengNature Publishing GroupNature Communications2041-17232019-08-011011910.1038/s41467-019-11648-1A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small moleculesMindong Liang0Zilong Li1Weishan Wang2Jiakun Liu3Leshi Liu4Guoliang Zhu5Loganathan Karthik6Man Wang7Ke-Feng Wang8Zhong Wang9Jing Yu10Yuting Shuai11Jiaming Yu12Lu Zhang13Zhiheng Yang14Chuan Li15Qian Zhang16Tong Shi17Liming Zhou18Feng Xie19Huanqin Dai20Xueting Liu21Jingyu Zhang22Guang Liu23Ying Zhuo24Buchang Zhang25Chenli Liu26Shanshan Li27Xuekui Xia28Yaojun Tong29Yanwen Liu30Gil Alterovitz31Gao-Yi Tan32Li-Xin Zhang33State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)School of Agriculture and Biology, Shanghai Jiao Tong UniversityState Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)Department of Urology, Sixth People’s Hospital South Campus Affiliated to Shanghai Jiao Tong UniversityDepartment of Clinical Laboratory, Sixth People’s Hospital South Campus Affiliated to Shanghai Jiao Tong UniversityState Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)Institute of Physical Science and Information Technology, Anhui UniversityShenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesKey Biosensor Laboratory of Shandong Province, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences)The Novo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkinBiome SciencesHarvard Medical School Countway LibraryState Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)Bacterial allosteric transcription factors can sense and respond to a variety of small molecules. Here the authors present CaT-SMelor which uses Cas12a and allosteric transcription factors to detect small molecules in the nanomolar range.https://doi.org/10.1038/s41467-019-11648-1 |