Development of an in-house HPLC method for the analysis of ecstasy-laced beverages
Abstract Background Pills of ecstasy continue to be the major drug of abuse among nightclub patrons. This stimulant type of substance can be taken orally in many ways, one of which is by drink spiking where the tablet or its powder is deliberately added to some beverage before consumption. Method A...
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doaj-41f55843dec8451f818aea2a1f741ac62020-11-25T00:00:45ZengSpringerOpenEgyptian Journal of Forensic Sciences2090-59392018-02-01811810.1186/s41935-018-0048-8Development of an in-house HPLC method for the analysis of ecstasy-laced beveragesKar-Weng Chan0Suraya Hani Ramli1Research and Instrumentation Unit, Department of Chemistry Malaysia, Ministry of Science, Technology and Innovation (MOSTI)Research and Instrumentation Unit, Department of Chemistry Malaysia, Ministry of Science, Technology and Innovation (MOSTI)Abstract Background Pills of ecstasy continue to be the major drug of abuse among nightclub patrons. This stimulant type of substance can be taken orally in many ways, one of which is by drink spiking where the tablet or its powder is deliberately added to some beverage before consumption. Method A high performance liquid chromatographic (HPLC) method was developed to quantify three analytes (methamphetamine, 3,4-methylenedioxymethamphetamine and ketamine) extracted from beverages associated with drink spiking. Ribena drink, Lipton tea drink and Pepsi soft drink were employed as the target sample matrices for validation studies. Results The HPLC method was found precise (RSD < 3.2%) and accurate (mean recovery between 97 and 107%) regardless of the matrix type used. The linearity curve for each analyte presented a good regression line with a coefficient of determination, R2 > 0.994. The method was sensitive to detect at least 0.004 mg/mL spiked drugs and precise to quantify the lowest limit of analytes at 0.02 mg/mL. Conclusion Analysis of beverages which were contrivedly spiked with genuine ecstasy substances substantiated that the method is fit for the intended purpose.http://link.springer.com/article/10.1186/s41935-018-0048-8EcstasyBeverageDrink spikingHPLC |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kar-Weng Chan Suraya Hani Ramli |
spellingShingle |
Kar-Weng Chan Suraya Hani Ramli Development of an in-house HPLC method for the analysis of ecstasy-laced beverages Egyptian Journal of Forensic Sciences Ecstasy Beverage Drink spiking HPLC |
author_facet |
Kar-Weng Chan Suraya Hani Ramli |
author_sort |
Kar-Weng Chan |
title |
Development of an in-house HPLC method for the analysis of ecstasy-laced beverages |
title_short |
Development of an in-house HPLC method for the analysis of ecstasy-laced beverages |
title_full |
Development of an in-house HPLC method for the analysis of ecstasy-laced beverages |
title_fullStr |
Development of an in-house HPLC method for the analysis of ecstasy-laced beverages |
title_full_unstemmed |
Development of an in-house HPLC method for the analysis of ecstasy-laced beverages |
title_sort |
development of an in-house hplc method for the analysis of ecstasy-laced beverages |
publisher |
SpringerOpen |
series |
Egyptian Journal of Forensic Sciences |
issn |
2090-5939 |
publishDate |
2018-02-01 |
description |
Abstract Background Pills of ecstasy continue to be the major drug of abuse among nightclub patrons. This stimulant type of substance can be taken orally in many ways, one of which is by drink spiking where the tablet or its powder is deliberately added to some beverage before consumption. Method A high performance liquid chromatographic (HPLC) method was developed to quantify three analytes (methamphetamine, 3,4-methylenedioxymethamphetamine and ketamine) extracted from beverages associated with drink spiking. Ribena drink, Lipton tea drink and Pepsi soft drink were employed as the target sample matrices for validation studies. Results The HPLC method was found precise (RSD < 3.2%) and accurate (mean recovery between 97 and 107%) regardless of the matrix type used. The linearity curve for each analyte presented a good regression line with a coefficient of determination, R2 > 0.994. The method was sensitive to detect at least 0.004 mg/mL spiked drugs and precise to quantify the lowest limit of analytes at 0.02 mg/mL. Conclusion Analysis of beverages which were contrivedly spiked with genuine ecstasy substances substantiated that the method is fit for the intended purpose. |
topic |
Ecstasy Beverage Drink spiking HPLC |
url |
http://link.springer.com/article/10.1186/s41935-018-0048-8 |
work_keys_str_mv |
AT karwengchan developmentofaninhousehplcmethodfortheanalysisofecstasylacedbeverages AT surayahaniramli developmentofaninhousehplcmethodfortheanalysisofecstasylacedbeverages |
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