Production of a mono-biotinylated EGFR nanobody in the E. coli periplasm using the pET22b vector

Production of a mono-biotinylated EGFR nanobody in the E. coli periplasm using the pET22b vector

Abstract Objective Our aim was to produce a mono-biotinylated single domain antibody (‘nanobody’) specific for the epidermal growth factor receptor (EGFR), which is overexpressed in many cancer cells. The binding of the nanobody and its function are tested in cancer cells. The construct could be use...

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Bibliographic Details
Main Authors: Alfiah Noor, Gudrun Walser, Matthijs Wesseling, Philippe Giron, Albert-Menno Laffra, Fatima Haddouchi, Jacques De Grève, Peter Kronenberger
Format: Article
Language:English
Published: BMC 2018-10-01
Series:BMC Research Notes
Subjects:
Nanocarriers
EGFR
Nanobody
Single-domain antibody
VHH domain
Mono-biotinylation
Online Access:http://link.springer.com/article/10.1186/s13104-018-3852-1

Internet

http://link.springer.com/article/10.1186/s13104-018-3852-1

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