Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue.

Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue.

Formalin-fixed, paraffin-embedded (FFPE) tissues are an invaluable resource for clinical research. However, nucleic acids extracted from FFPE tissues are fragmented and chemically modified making them challenging to use in molecular studies. We analysed 23 fresh-frozen (FF), 35 FFPE and 38 paired FF...

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Main Authors: Jakob Hedegaard, Kasper Thorsen, Mette Katrine Lund, Anne-Mette K Hein, Stephen Jacques Hamilton-Dutoit, Søren Vang, Iver Nordentoft, Karin Birkenkamp-Demtröder, Mogens Kruhøffer, Henrik Hager, Bjarne Knudsen, Claus Lindbjerg Andersen, Karina Dalsgaard Sørensen, Jakob Skou Pedersen, Torben Falck Ørntoft, Lars Dyrskjøt
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4039489?pdf=render

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