Attenuation of Mycoplasma hyopneumoniae Strain ES-2 and Comparative Genomic Analysis of ES-2 and Its Attenuated Form ES-2L
Mycoplasma hyopneumoniae causes swine respiratory disease worldwide. Due to the difficulty of isolating and cultivating M. hyopneumoniae, very few attenuated strains have been successfully isolated, which hampers the development of attenuated vaccines. In order to produce an attenuated M. hyopneumon...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2021-06-01
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Series: | Frontiers in Veterinary Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.696262/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhenya Li Zhenya Li Yingxin Wang Yingxin Wang Yanyan Zhang Yanyan Zhang Xibiao Tang Xibiao Tang Xibiao Tang Xiangru Wang Xiangru Wang Wenhao Liu Wenhao Liu Yulin Qian Yulin Qian Yongwei Zhu Yongwei Zhu Huanchun Chen Huanchun Chen Huanchun Chen Huanchun Chen Chen Tan Chen Tan Chen Tan |
spellingShingle |
Zhenya Li Zhenya Li Yingxin Wang Yingxin Wang Yanyan Zhang Yanyan Zhang Xibiao Tang Xibiao Tang Xibiao Tang Xiangru Wang Xiangru Wang Wenhao Liu Wenhao Liu Yulin Qian Yulin Qian Yongwei Zhu Yongwei Zhu Huanchun Chen Huanchun Chen Huanchun Chen Huanchun Chen Chen Tan Chen Tan Chen Tan Attenuation of Mycoplasma hyopneumoniae Strain ES-2 and Comparative Genomic Analysis of ES-2 and Its Attenuated Form ES-2L Frontiers in Veterinary Science Mycoplasma hyopneumoniae virulence attenuation in vitro passaging sequence analysis |
author_facet |
Zhenya Li Zhenya Li Yingxin Wang Yingxin Wang Yanyan Zhang Yanyan Zhang Xibiao Tang Xibiao Tang Xibiao Tang Xiangru Wang Xiangru Wang Wenhao Liu Wenhao Liu Yulin Qian Yulin Qian Yongwei Zhu Yongwei Zhu Huanchun Chen Huanchun Chen Huanchun Chen Huanchun Chen Chen Tan Chen Tan Chen Tan |
author_sort |
Zhenya Li |
title |
Attenuation of Mycoplasma hyopneumoniae Strain ES-2 and Comparative Genomic Analysis of ES-2 and Its Attenuated Form ES-2L |
title_short |
Attenuation of Mycoplasma hyopneumoniae Strain ES-2 and Comparative Genomic Analysis of ES-2 and Its Attenuated Form ES-2L |
title_full |
Attenuation of Mycoplasma hyopneumoniae Strain ES-2 and Comparative Genomic Analysis of ES-2 and Its Attenuated Form ES-2L |
title_fullStr |
Attenuation of Mycoplasma hyopneumoniae Strain ES-2 and Comparative Genomic Analysis of ES-2 and Its Attenuated Form ES-2L |
title_full_unstemmed |
Attenuation of Mycoplasma hyopneumoniae Strain ES-2 and Comparative Genomic Analysis of ES-2 and Its Attenuated Form ES-2L |
title_sort |
attenuation of mycoplasma hyopneumoniae strain es-2 and comparative genomic analysis of es-2 and its attenuated form es-2l |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2021-06-01 |
description |
Mycoplasma hyopneumoniae causes swine respiratory disease worldwide. Due to the difficulty of isolating and cultivating M. hyopneumoniae, very few attenuated strains have been successfully isolated, which hampers the development of attenuated vaccines. In order to produce an attenuated M. hyopneumoniae strain, we used the highly virulent M. hyopneumoniae strain ES-2, which was serially passaged in vitro 200 times to produce the attenuated strain ES-2L, and its virulence was evidenced to be low in an animal experiment. In order to elucidate the mechanisms underlying virulence attenuation, we performed whole-genome sequencing of both strains and conducted comparative genomic analyses of strain ES-2 and its attenuated form ES-2L. Strain ES-2L showed three large fragment deletion regions including a total of 18 deleted genes, compared with strain ES-2. Analysis of single-nucleotide polymorphisms (SNPs) and indels indicated that 22 dels were located in 19 predicted coding sequences. In addition to these indels, 348 single-nucleotide variations (SNVs) were identified between strains ES-2L and ES-2. These SNVs mapped to 99 genes where they appeared to induce amino acid substitutions and translation stops. The deleted genes and SNVs may be associated with decreased virulence of strain ES-2L. Our work provides a foundation for further examining virulence factors of M. hyopneumoniae and for the development of attenuated vaccines. |
topic |
Mycoplasma hyopneumoniae virulence attenuation in vitro passaging sequence analysis |
url |
https://www.frontiersin.org/articles/10.3389/fvets.2021.696262/full |
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doaj-41cb65d35e81476f9038a6d5f37a88842021-06-21T05:40:02ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-06-01810.3389/fvets.2021.696262696262Attenuation of Mycoplasma hyopneumoniae Strain ES-2 and Comparative Genomic Analysis of ES-2 and Its Attenuated Form ES-2LZhenya Li0Zhenya Li1Yingxin Wang2Yingxin Wang3Yanyan Zhang4Yanyan Zhang5Xibiao Tang6Xibiao Tang7Xibiao Tang8Xiangru Wang9Xiangru Wang10Wenhao Liu11Wenhao Liu12Yulin Qian13Yulin Qian14Yongwei Zhu15Yongwei Zhu16Huanchun Chen17Huanchun Chen18Huanchun Chen19Huanchun Chen20Chen Tan21Chen Tan22Chen Tan23State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaWuhan Keqian Biology Co., Ltd., Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaWuhan Keqian Biology Co., Ltd., Wuhan, ChinaInternational Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, ChinaInternational Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, ChinaMycoplasma hyopneumoniae causes swine respiratory disease worldwide. Due to the difficulty of isolating and cultivating M. hyopneumoniae, very few attenuated strains have been successfully isolated, which hampers the development of attenuated vaccines. In order to produce an attenuated M. hyopneumoniae strain, we used the highly virulent M. hyopneumoniae strain ES-2, which was serially passaged in vitro 200 times to produce the attenuated strain ES-2L, and its virulence was evidenced to be low in an animal experiment. In order to elucidate the mechanisms underlying virulence attenuation, we performed whole-genome sequencing of both strains and conducted comparative genomic analyses of strain ES-2 and its attenuated form ES-2L. Strain ES-2L showed three large fragment deletion regions including a total of 18 deleted genes, compared with strain ES-2. Analysis of single-nucleotide polymorphisms (SNPs) and indels indicated that 22 dels were located in 19 predicted coding sequences. In addition to these indels, 348 single-nucleotide variations (SNVs) were identified between strains ES-2L and ES-2. These SNVs mapped to 99 genes where they appeared to induce amino acid substitutions and translation stops. The deleted genes and SNVs may be associated with decreased virulence of strain ES-2L. Our work provides a foundation for further examining virulence factors of M. hyopneumoniae and for the development of attenuated vaccines.https://www.frontiersin.org/articles/10.3389/fvets.2021.696262/fullMycoplasma hyopneumoniaevirulenceattenuationin vitro passagingsequence analysis |