Correlation of Gab2 and IRX2 expression with cell growth and migration behavior in osteosarcoma tissues

• Main Author: Xue-Jun Zhou
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-12-01
• Series: Journal of Hainan Medical University
• Subjects: Osteosarcoma; Gab2; IRX2; Proliferation; Apoptosis; Migration
• Online Access: http://www.hnykdxxb.com/PDF/201624/3.pdf
• ISSN: 1007-1237; 1007-1237

Objective: To study the correlation of Gab2 and IRX2 expression with cell growth and migration behavior in osteosarcoma tissues. Methods: A total of 29 cases of osteosarcoma tissues and 22 cases of osteoid osteoma tissues surgically removed in our hospital between May 2012 and December 2015 were selected as the test specimens, RNA was extracted to determine the mRNA expression of Gab2 and IRX2 as well as cell growth- and migration-related genes, and protein was extracted to determine the protein expression of Gab2 and IRX2 as well as cell growth- and migration-related genes. Results: Gab2 and IRX2 mRNA content and protein content in osteosarcoma tissues were significantly higher than those in osteoid osteoma tissues; TAp73, Beclin1 and Caspase-1 mRNA content and protein content in osteosarcoma tissues were significantly lower than those in osteoid osteoma tissues and negatively correlated with Gab2 and IRX2 mRNA content and protein content while STMN1 and Survivin mRNA content and protein content were significantly higher than those in osteoid osteoma tissues and positively correlated with Gab2, IRX2, CEACAM6, CD44v6 and MMP-9 mRNA content and protein content. Conclusions: Highly expressed Gab2 and IRX2 in osteosarcoma tissue can inhibit the expression of proto-oncogenes and increase the expression of proliferation and migration genes to promote the cell growth and migration in osteosarcoma tissue.