QUANTITATIVE AND FUNCTIONAL CHARACTERISTICS OF RAT INTESTINAL MICROBIOTA

• Main Authors: А. В. Путніков, Ю. В. Голота, Т. М. Сергійчук, А. М. Остапчук, Л. В. Закордонець, Л. І. Остапченко, Г. М. Толстанова
• Format: Article
• Language: English
• Published: Odessa I. I. Mechnikov National University 2015-06-01
• Series: Mìkrobìologìâ ì Bìotehnologìâ
• Subjects: нормобіота; референс-діапазони; коротколанцюгові жирні кислоти; тонка кишка; товста кишка
• Online Access: http://mbt.onu.edu.ua/article/view/48083

Aim. The aim of this study was to determine reference ranges of microbiological parameters of laboratory rats faecal and parietal intestinal biotope and a comparative analysis of existing data for the human indicators. Methods. Study was done on male Wistar rats (170–200 g, n=31). The fecal (CFU/g) and parietal (CFU/cm2) microbiota have been analyzed by bacteriological culture methods, levels of SCFA by gas chromatography. Results. Rat feces contained 106–109 CFU/g, Bifidobacterium sp. 105–109 CFU/g, Propionibacterium sp. 105–109 CFU/g and Lactobacillus 106–108 CFU/g. There were determined almost equal ratio between lactose (+) E. coli (104–107 CFU/g) and lactose (-) E. coli (102–105 CFU/g ). Quantity of conditionally- pathogenic bacteria (Citrobacter, Klebsiella, Enterobacter, etc.) did not exceed 103 CFU/g , Clostridium sp. – 102–104 CFU/g, Staphylococcus sp. – 0–107 CFU/g , S. aureus – 103–105 CFU/g, Candida spp. – 0–103 CFU/g. Hemolytics trains were not detected. Mucosa of the colon andthe terminalileum was not colonized by Clostridium spp. and Candida spp. But 100% of investigated animals were colonized with Bifidobacterium (103–106 CFU/ cm2), Lactobacillus (102–106 CFU/cm2), Propionibacterium (103–106 CFU/cm2). The composition of rat’s SCFU in faeces is similar to human’s and contains: acetic (C2), propionic (C3), butyric and isobutyric (C4 iS4) valeric and isovaleric (C5 iC5) hexanoic and isocaproic (C6 iC6) acids. Conclusion. Wistar rats were determined with reference ranges of major groups of microorganisms fecal biopsy, which are taken into account when assessing the state of human intestinal normobioty. For the first time reference ranges of rats colon and small intestine parietal microbiota biopsy are defined. The ratio of SCFA of Wistar rats feces (C2: C3: C4) is 41.9: 32.2: 20.9%.