Osmotic stress tolerance, PGP traits and RAPD analysis of Bradyrhizobium japonicum strains

• Main Authors: Marinković Jelena, Đorđević Vuk, Balešević-Tubić Svetlana, Bjelić Dragana, Vucelić-Radović Biljana, Jošić Dragana
• Format: Article
• Language: English
• Published: Serbian Genetics Society 2013-01-01
• Series: Genetika
• Subjects: Bradyrhizobium japonicum; osmotic stress tolerance; RAPD analysis; PGPR
• Online Access: http://www.doiserbia.nb.rs/img/doi/0534-0012/2013/0534-00121301075M.pdf

The osmotic stress tolerance of B. japonicum strains assessed according to their persistence in PEG solution. The lowest tolerance to osmotic stress was observed in strain 511 (43.3%), and the highest tolerance was observed for strain D216 (3.3% growth reduction in presence of PEG). PGP traits of B. japonicum strains were tested. None of five B. japonicum strains produced siderophore, strains 511 and 518 had the urease ability, and only B. japonicum 518 strain showed the ability to solubilize insoluble tricalcium phosphate. RAPD analysis, using AP10, BC318, AF14 and SPH1 primers, indicated genetic differences between Bradyrhizobium strains. The first group (strains 3, 6 and 518) showed more than 80% similarity. Strains 511 and D216 formed separate clusters. Difference between strains D216 and the other strains were more than 60%, with maximum value of 72% in comparison with strain 511. Plant-growth promoting (PGP) traits, osmotic stress tolerance and RAPD analysis highlighted strain D216 as useful for further investigation of B. japonicum impact on drought reduction in symbiosis with soybean. [Projekat Ministarstva nauke Republike Srbije, br. TR 31022 i br. TR 31072]