PCR-based gene synthesis to produce recombinant proteins for crystallization

<p>Abstract</p> <p>Background</p> <p>Gene synthesis technologies are an important tool for structural biology projects, allowing increased protein expression through codon optimization and facilitating sequence alterations. Existing methods, however, can be complex and...

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Bibliographic Details
Main Authors: Byrne-Steele Miranda L, Hughes Ronny C, Marsic Damien, Ng Joseph D
Format: Article
Language:English
Published: BMC 2008-04-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/8/44