The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration

The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration

The voltage-dependent anion channel (VDAC) is the major pathway for metabolites and ions transport through the mitochondrial outer membrane. It can regulate the flow of solutes by switching to a low conductance state correlated with a selectivity reversal, or by a selectivity inversion of its open s...

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Main Authors: Hayet Saidani, Marc Léonetti, Hanna Kmita, Fabrice Homblé
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:International Journal of Molecular Sciences
Subjects:
VDAC
selectivity
mitochondria
protein-lipid interaction
stigmasterol
phaseolus
Online Access:https://www.mdpi.com/1422-0067/22/6/3034
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spelling doaj-40f91b93ce2c4165ad8246a4bfd4fd122021-03-17T00:05:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01223034303410.3390/ijms22063034The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion ConcentrationHayet Saidani0Marc Léonetti1Hanna Kmita2Fabrice Homblé3Structure et Fonction des Membranes Biologiques, Université Libre de Bruxelles (ULB), Boulevard du Triomphe CP 206/2, B-1050 Bruxelles, BelgiumUniversité de. Grenoble Alpes, CNRS, LRP, 38000 Grenoble, FranceDepartment of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, 61-614 Poznań, PolandStructure et Fonction des Membranes Biologiques, Université Libre de Bruxelles (ULB), Boulevard du Triomphe CP 206/2, B-1050 Bruxelles, BelgiumThe voltage-dependent anion channel (VDAC) is the major pathway for metabolites and ions transport through the mitochondrial outer membrane. It can regulate the flow of solutes by switching to a low conductance state correlated with a selectivity reversal, or by a selectivity inversion of its open state. The later one was observed in non-plant VDACs and is poorly characterized. We aim at investigating the selectivity inversion of the open state using plant VDAC purified from <i>Phaseolus coccineus</i> (PcVDAC) to evaluate its physiological role. Our main findings are: (1) The VDAC selectivity inversion of the open state occurs in PcVDAC, (2) Ion concentration and stigmasterol affect the occurrence of the open state selectivity inversion and stigmasterol appears to interact directly with PcVDAC. Interestingly, electrophysiological data concerning the selectivity inversion of the PcVDAC open state suggests that the phenomenon probably does not have a significant physiological effect in vivo.https://www.mdpi.com/1422-0067/22/6/3034VDACselectivitymitochondriaprotein-lipid interactionstigmasterolphaseolus
collection DOAJ
language English
format Article
sources DOAJ
author Hayet Saidani
Marc Léonetti
Hanna Kmita
Fabrice Homblé
spellingShingle Hayet Saidani
Marc Léonetti
Hanna Kmita
Fabrice Homblé
The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration
International Journal of Molecular Sciences
VDAC
selectivity
mitochondria
protein-lipid interaction
stigmasterol
phaseolus
author_facet Hayet Saidani
Marc Léonetti
Hanna Kmita
Fabrice Homblé
author_sort Hayet Saidani
title The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration
title_short The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration
title_full The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration
title_fullStr The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration
title_full_unstemmed The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration
title_sort open state selectivity of the bean seed vdac depends on stigmasterol and ion concentration
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2021-03-01
description The voltage-dependent anion channel (VDAC) is the major pathway for metabolites and ions transport through the mitochondrial outer membrane. It can regulate the flow of solutes by switching to a low conductance state correlated with a selectivity reversal, or by a selectivity inversion of its open state. The later one was observed in non-plant VDACs and is poorly characterized. We aim at investigating the selectivity inversion of the open state using plant VDAC purified from <i>Phaseolus coccineus</i> (PcVDAC) to evaluate its physiological role. Our main findings are: (1) The VDAC selectivity inversion of the open state occurs in PcVDAC, (2) Ion concentration and stigmasterol affect the occurrence of the open state selectivity inversion and stigmasterol appears to interact directly with PcVDAC. Interestingly, electrophysiological data concerning the selectivity inversion of the PcVDAC open state suggests that the phenomenon probably does not have a significant physiological effect in vivo.
topic VDAC
selectivity
mitochondria
protein-lipid interaction
stigmasterol
phaseolus
url https://www.mdpi.com/1422-0067/22/6/3034
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