A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.

A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.

DEAE-silica gel has been shown to be an improvement over DEAE-Sephadex for the quantitative isolation of gangliosides and neutral glycosphingolipids from animal tissues or cells. Preliminary results indicated that it can also be used for protein separation. Direct comparative studies of DEAE-silica...

Full description

Bibliographic Details
Main Authors: S K Kundu, S K Roy
Format: Article
Language:English
Published: Elsevier 1978-03-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520413136
id doaj-40da5ad001604f8891da1944fcd4c47e
record_format Article
spelling doaj-40da5ad001604f8891da1944fcd4c47e2021-04-24T05:53:34ZengElsevierJournal of Lipid Research0022-22751978-03-01193390395A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.S K KunduS K RoyDEAE-silica gel has been shown to be an improvement over DEAE-Sephadex for the quantitative isolation of gangliosides and neutral glycosphingolipids from animal tissues or cells. Preliminary results indicated that it can also be used for protein separation. Direct comparative studies of DEAE-silica gel with DEAE-Sephadex showed preferences for the former for the following reasons: i) faster flow rate; ii) more rapid equilibration with the starting buffer; iii) easier regeneration; iv) more economical; and v) a lesser susceptibility to microbial attack.http://www.sciencedirect.com/science/article/pii/S0022227520413136
collection DOAJ
language English
format Article
sources DOAJ
author S K Kundu
S K Roy
spellingShingle S K Kundu
S K Roy
A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.
Journal of Lipid Research
author_facet S K Kundu
S K Roy
author_sort S K Kundu
title A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.
title_short A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.
title_full A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.
title_fullStr A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.
title_full_unstemmed A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.
title_sort rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by deae-silica gel chromatography.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1978-03-01
description DEAE-silica gel has been shown to be an improvement over DEAE-Sephadex for the quantitative isolation of gangliosides and neutral glycosphingolipids from animal tissues or cells. Preliminary results indicated that it can also be used for protein separation. Direct comparative studies of DEAE-silica gel with DEAE-Sephadex showed preferences for the former for the following reasons: i) faster flow rate; ii) more rapid equilibration with the starting buffer; iii) easier regeneration; iv) more economical; and v) a lesser susceptibility to microbial attack.
url http://www.sciencedirect.com/science/article/pii/S0022227520413136
work_keys_str_mv AT skkundu arapidandquantitativemethodfortheisolationofgangliosidesandneutralglycosphingolipidsbydeaesilicagelchromatography
AT skroy arapidandquantitativemethodfortheisolationofgangliosidesandneutralglycosphingolipidsbydeaesilicagelchromatography
AT skkundu rapidandquantitativemethodfortheisolationofgangliosidesandneutralglycosphingolipidsbydeaesilicagelchromatography
AT skroy rapidandquantitativemethodfortheisolationofgangliosidesandneutralglycosphingolipidsbydeaesilicagelchromatography
_version_ 1721511441928814592

Similar Items