Improvised Media for In Vitro Pollen Germination of some Species of Apocynaceae

<p class="Default">Pollen germination forms one of the most important stage post pollination prior to fertilization. This is essential for proper seed setting and seed development. <em>In vitro </em>pollen germination test is the most reliable way of assessing the pollen...

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Bibliographic Details
Main Authors: Reshmi Chatterjee, Satadip Sarkar, GM Narasimha Rao
Format: Article
Language:English
Published: Progressive Sustainable Developers Nepal 2014-09-01
Series:International Journal of Environment
Subjects:
Online Access:http://nepjol.info/index.php/IJE/article/view/11074
Description
Summary:<p class="Default">Pollen germination forms one of the most important stage post pollination prior to fertilization. This is essential for proper seed setting and seed development. <em>In vitro </em>pollen germination test is the most reliable way of assessing the pollen viability. In the present study pollen grains of seven genera under Apocynaceae family namely, <em>Allamanda</em>, <em>Alstonia</em>, <em>Catharanthus</em>, <em>Nerium, Plumeria</em>, <em>Thevetia </em>and <em>Tabernaemontana </em>were tested in some basic cultural media, such as Brewbaker&rsquo;s media, 6% Glucose solution, 4% Calcium Nitrate solution and 3% Boron solution. <em>Alstonia </em>pollen grains exhibited highest percentage of germination rate in all the cultural media. Glucose and Brewbaker&rsquo;s media is found to be highly suitable for efficient pollen germination in all the genera. Boron solution is effective for germination of pollen grains of tree species. <em>In vitro </em>pollen germination can be easily carried out in laboratories. These results can be utilised in plant breeding programmes to improve cultivar and varieties.</p> <p>DOI: <a href="http://dx.doi.org/10.3126/ije.v3i3.11074">http://dx.doi.org/10.3126/ije.v3i3.11074</a></p> <p>International Journal of Environment Vol.3(3) 2014: 146-153</p>
ISSN:2091-2854