Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant

A naturally occurring yellow tomato fruit mutant cv. Santa Clara was reciprocally crossed with the red wild type, after which F1 plants were self pollinated or backcrossed with both parents. Plants from F1 generations produced all fruits with a homogeneous deep red color when ripe. F2 plants showed...

Full description

Bibliographic Details
Main Authors: Elizanilda R. do Rêgo, Fernando L. Finger, Vicente W.D. Casali, Antônio A. Cardoso
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 1999-03-01
Series:Genetics and Molecular Biology
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100019
id doaj-40abd6487460492a9fe2c672b34c7d7e
record_format Article
spelling doaj-40abd6487460492a9fe2c672b34c7d7e2020-11-25T01:25:20ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46851999-03-0122110110410.1590/S1415-47571999000100019Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutantElizanilda R. do RêgoFernando L. FingerVicente W.D. CasaliAntônio A. CardosoA naturally occurring yellow tomato fruit mutant cv. Santa Clara was reciprocally crossed with the red wild type, after which F1 plants were self pollinated or backcrossed with both parents. Plants from F1 generations produced all fruits with a homogeneous deep red color when ripe. F2 plants showed a 3:1 red:yellow segregation of fruit color, and 100% red when backcrossed with red wild type or 1:1 red:yellow segregation in backcrosses with the yellow mutant; hence, yellow fruit color was determined by a recessive allele. Based on reciprocal crosses, fruit color is unlikely to be determined by maternal genes. Accumulation of lycopene dropped by 99.3% and<FONT FACE="Symbol"> b</font>-carotene by 77% in ripe yellow fruits, compared to the red wild type. Leaf and flower chlorophyll and total carotenoid concentrations were not affected by the yellow mutation. However, the mutant fruit had a higher rate of chlorophyll degradation during fruit ripening, whilst fruit from the F1 generation showed lower rates of degradation, similar to that observed in red wild type fruits.<br>Neste trabalho avaliou-se a herança da cor do fruto de um mutante natural da cv. Santa Clara, por meio da análise das gerações F1 e segregantes, obtidas mediante cruzamento entre plantas da cv. Santa Clara normal e o mutante amarelo. A caracterização das plantas normais, mutantes e F1 foi feita com base na análise quantitativa dos pigmentos carotenóides e clorofila em flores, folhas e frutos verdes e maduros. Plantas F1 e provenientes do retrocruzamento com o progenitor normal apresentaram 100% de frutos vermelhos. A semelhança entre os F1 recíprocos mostra que há ausência de herança materna para as características avaliadas. Em gerações segregantes, as freqüências observadas foram compatíveis com herança monogênica pelo teste qui-quadrado, com dominância completa para o gene que confere cor vermelha. Os frutos amarelos apresentaram teores reduzidos de <FONT FACE="Symbol">b</font>-caroteno e licopeno, enquanto o híbrido apresentou teores intermediários desses carotenóides quando comparados com o genótipo normal. Os níveis de clorofila em frutos verde-maduros e maduros mutantes foram menores que nos frutos normais, evidenciando o papel dos carotenóides sobre a fotoproteção da clorofila. A concentração de clorofila e carotenóides, em folhas e flores, não foi afetada pela mutação.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100019
collection DOAJ
language English
format Article
sources DOAJ
author Elizanilda R. do Rêgo
Fernando L. Finger
Vicente W.D. Casali
Antônio A. Cardoso
spellingShingle Elizanilda R. do Rêgo
Fernando L. Finger
Vicente W.D. Casali
Antônio A. Cardoso
Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant
Genetics and Molecular Biology
author_facet Elizanilda R. do Rêgo
Fernando L. Finger
Vicente W.D. Casali
Antônio A. Cardoso
author_sort Elizanilda R. do Rêgo
title Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant
title_short Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant
title_full Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant
title_fullStr Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant
title_full_unstemmed Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant
title_sort inheritance of fruit color and pigment changes in a yellow tomato (lycopersicon esculentum mill.) mutant
publisher Sociedade Brasileira de Genética
series Genetics and Molecular Biology
issn 1415-4757
1678-4685
publishDate 1999-03-01
description A naturally occurring yellow tomato fruit mutant cv. Santa Clara was reciprocally crossed with the red wild type, after which F1 plants were self pollinated or backcrossed with both parents. Plants from F1 generations produced all fruits with a homogeneous deep red color when ripe. F2 plants showed a 3:1 red:yellow segregation of fruit color, and 100% red when backcrossed with red wild type or 1:1 red:yellow segregation in backcrosses with the yellow mutant; hence, yellow fruit color was determined by a recessive allele. Based on reciprocal crosses, fruit color is unlikely to be determined by maternal genes. Accumulation of lycopene dropped by 99.3% and<FONT FACE="Symbol"> b</font>-carotene by 77% in ripe yellow fruits, compared to the red wild type. Leaf and flower chlorophyll and total carotenoid concentrations were not affected by the yellow mutation. However, the mutant fruit had a higher rate of chlorophyll degradation during fruit ripening, whilst fruit from the F1 generation showed lower rates of degradation, similar to that observed in red wild type fruits.<br>Neste trabalho avaliou-se a herança da cor do fruto de um mutante natural da cv. Santa Clara, por meio da análise das gerações F1 e segregantes, obtidas mediante cruzamento entre plantas da cv. Santa Clara normal e o mutante amarelo. A caracterização das plantas normais, mutantes e F1 foi feita com base na análise quantitativa dos pigmentos carotenóides e clorofila em flores, folhas e frutos verdes e maduros. Plantas F1 e provenientes do retrocruzamento com o progenitor normal apresentaram 100% de frutos vermelhos. A semelhança entre os F1 recíprocos mostra que há ausência de herança materna para as características avaliadas. Em gerações segregantes, as freqüências observadas foram compatíveis com herança monogênica pelo teste qui-quadrado, com dominância completa para o gene que confere cor vermelha. Os frutos amarelos apresentaram teores reduzidos de <FONT FACE="Symbol">b</font>-caroteno e licopeno, enquanto o híbrido apresentou teores intermediários desses carotenóides quando comparados com o genótipo normal. Os níveis de clorofila em frutos verde-maduros e maduros mutantes foram menores que nos frutos normais, evidenciando o papel dos carotenóides sobre a fotoproteção da clorofila. A concentração de clorofila e carotenóides, em folhas e flores, não foi afetada pela mutação.
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100019
work_keys_str_mv AT elizanildardorego inheritanceoffruitcolorandpigmentchangesinayellowtomatolycopersiconesculentummillmutant
AT fernandolfinger inheritanceoffruitcolorandpigmentchangesinayellowtomatolycopersiconesculentummillmutant
AT vicentewdcasali inheritanceoffruitcolorandpigmentchangesinayellowtomatolycopersiconesculentummillmutant
AT antonioacardoso inheritanceoffruitcolorandpigmentchangesinayellowtomatolycopersiconesculentummillmutant
_version_ 1725114592671039488