Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant
A naturally occurring yellow tomato fruit mutant cv. Santa Clara was reciprocally crossed with the red wild type, after which F1 plants were self pollinated or backcrossed with both parents. Plants from F1 generations produced all fruits with a homogeneous deep red color when ripe. F2 plants showed...
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Sociedade Brasileira de Genética
1999-03-01
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doaj-40abd6487460492a9fe2c672b34c7d7e2020-11-25T01:25:20ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46851999-03-0122110110410.1590/S1415-47571999000100019Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutantElizanilda R. do RêgoFernando L. FingerVicente W.D. CasaliAntônio A. CardosoA naturally occurring yellow tomato fruit mutant cv. Santa Clara was reciprocally crossed with the red wild type, after which F1 plants were self pollinated or backcrossed with both parents. Plants from F1 generations produced all fruits with a homogeneous deep red color when ripe. F2 plants showed a 3:1 red:yellow segregation of fruit color, and 100% red when backcrossed with red wild type or 1:1 red:yellow segregation in backcrosses with the yellow mutant; hence, yellow fruit color was determined by a recessive allele. Based on reciprocal crosses, fruit color is unlikely to be determined by maternal genes. Accumulation of lycopene dropped by 99.3% and<FONT FACE="Symbol"> b</font>-carotene by 77% in ripe yellow fruits, compared to the red wild type. Leaf and flower chlorophyll and total carotenoid concentrations were not affected by the yellow mutation. However, the mutant fruit had a higher rate of chlorophyll degradation during fruit ripening, whilst fruit from the F1 generation showed lower rates of degradation, similar to that observed in red wild type fruits.<br>Neste trabalho avaliou-se a herança da cor do fruto de um mutante natural da cv. Santa Clara, por meio da análise das gerações F1 e segregantes, obtidas mediante cruzamento entre plantas da cv. Santa Clara normal e o mutante amarelo. A caracterização das plantas normais, mutantes e F1 foi feita com base na análise quantitativa dos pigmentos carotenóides e clorofila em flores, folhas e frutos verdes e maduros. Plantas F1 e provenientes do retrocruzamento com o progenitor normal apresentaram 100% de frutos vermelhos. A semelhança entre os F1 recíprocos mostra que há ausência de herança materna para as características avaliadas. Em gerações segregantes, as freqüências observadas foram compatíveis com herança monogênica pelo teste qui-quadrado, com dominância completa para o gene que confere cor vermelha. Os frutos amarelos apresentaram teores reduzidos de <FONT FACE="Symbol">b</font>-caroteno e licopeno, enquanto o híbrido apresentou teores intermediários desses carotenóides quando comparados com o genótipo normal. Os níveis de clorofila em frutos verde-maduros e maduros mutantes foram menores que nos frutos normais, evidenciando o papel dos carotenóides sobre a fotoproteção da clorofila. A concentração de clorofila e carotenóides, em folhas e flores, não foi afetada pela mutação.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100019 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elizanilda R. do Rêgo Fernando L. Finger Vicente W.D. Casali Antônio A. Cardoso |
spellingShingle |
Elizanilda R. do Rêgo Fernando L. Finger Vicente W.D. Casali Antônio A. Cardoso Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant Genetics and Molecular Biology |
author_facet |
Elizanilda R. do Rêgo Fernando L. Finger Vicente W.D. Casali Antônio A. Cardoso |
author_sort |
Elizanilda R. do Rêgo |
title |
Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant |
title_short |
Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant |
title_full |
Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant |
title_fullStr |
Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant |
title_full_unstemmed |
Inheritance of fruit color and pigment changes in a yellow tomato (Lycopersicon esculentum Mill.) mutant |
title_sort |
inheritance of fruit color and pigment changes in a yellow tomato (lycopersicon esculentum mill.) mutant |
publisher |
Sociedade Brasileira de Genética |
series |
Genetics and Molecular Biology |
issn |
1415-4757 1678-4685 |
publishDate |
1999-03-01 |
description |
A naturally occurring yellow tomato fruit mutant cv. Santa Clara was reciprocally crossed with the red wild type, after which F1 plants were self pollinated or backcrossed with both parents. Plants from F1 generations produced all fruits with a homogeneous deep red color when ripe. F2 plants showed a 3:1 red:yellow segregation of fruit color, and 100% red when backcrossed with red wild type or 1:1 red:yellow segregation in backcrosses with the yellow mutant; hence, yellow fruit color was determined by a recessive allele. Based on reciprocal crosses, fruit color is unlikely to be determined by maternal genes. Accumulation of lycopene dropped by 99.3% and<FONT FACE="Symbol"> b</font>-carotene by 77% in ripe yellow fruits, compared to the red wild type. Leaf and flower chlorophyll and total carotenoid concentrations were not affected by the yellow mutation. However, the mutant fruit had a higher rate of chlorophyll degradation during fruit ripening, whilst fruit from the F1 generation showed lower rates of degradation, similar to that observed in red wild type fruits.<br>Neste trabalho avaliou-se a herança da cor do fruto de um mutante natural da cv. Santa Clara, por meio da análise das gerações F1 e segregantes, obtidas mediante cruzamento entre plantas da cv. Santa Clara normal e o mutante amarelo. A caracterização das plantas normais, mutantes e F1 foi feita com base na análise quantitativa dos pigmentos carotenóides e clorofila em flores, folhas e frutos verdes e maduros. Plantas F1 e provenientes do retrocruzamento com o progenitor normal apresentaram 100% de frutos vermelhos. A semelhança entre os F1 recíprocos mostra que há ausência de herança materna para as características avaliadas. Em gerações segregantes, as freqüências observadas foram compatíveis com herança monogênica pelo teste qui-quadrado, com dominância completa para o gene que confere cor vermelha. Os frutos amarelos apresentaram teores reduzidos de <FONT FACE="Symbol">b</font>-caroteno e licopeno, enquanto o híbrido apresentou teores intermediários desses carotenóides quando comparados com o genótipo normal. Os níveis de clorofila em frutos verde-maduros e maduros mutantes foram menores que nos frutos normais, evidenciando o papel dos carotenóides sobre a fotoproteção da clorofila. A concentração de clorofila e carotenóides, em folhas e flores, não foi afetada pela mutação. |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100019 |
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