Sucrose synthase molecular marker associated with sugar content in elite sugarcane progeny

• Main Authors: Jorge A. da Silva, Jose A. Bressiani
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Genética 2005-01-01
• Series: Genetics and Molecular Biology
• Subjects: sucrose; synthase; expressed; sequence; tag
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200020

We describe the development and application of an expressed sequence tag (EST)-derived restriction fragment length polymorphism (RFLP) marker for sugarcane elite genotypes which can be used for quantitative trait loci (QTL) tagging for sugar content. EST-derived RFLP markers for proteins involved in sucrose metabolism have been used in Southern analysis for mapping and gene tagging in elite sugarcane clones. A single dose marker, obtained from a sucrose synthase EST associated with sugar content at the alpha = 0.01 probability level, is presented for sugarcane breeding. Utilization of EST homologues to known genes for generation of molecular markers accelerated the identification of a QTL controlling an important trait-sugar content. Sugarcane bacterial artificial chromosome (BAC) clones hybridizing to the sucrose synthase EST were identified.