Quality control on the frontier
In the world of high-throughput sequencing there are numerous challenges to effective data quality control. There are no single quality metrics which are appropriate in all conditions. Here we detail the different open source software used at the Exeter Sequencing Service to provide generic quality...
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Frontiers Media S.A.
2014-05-01
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| Series: | Frontiers in Genetics |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00157/full |
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doaj-408557fbf32f46f2980b06ca22b0ff282020-11-25T00:12:31ZengFrontiers Media S.A.Frontiers in Genetics1664-80212014-05-01510.3389/fgene.2014.0015777173Quality control on the frontierKonrad Hubert Paszkiewicz0Audrey eFarbos1Paul eO'Neill2Karen eMoore3University of ExeterUniversity of ExeterUniversity of ExeterUniversity of ExeterIn the world of high-throughput sequencing there are numerous challenges to effective data quality control. There are no single quality metrics which are appropriate in all conditions. Here we detail the different open source software used at the Exeter Sequencing Service to provide generic quality control information, as well as more specific metrics for genomic and transcriptomic libraries run on Illumina platforms.http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00157/fullSequencingQuality controlIlluminacontaminationCore-facilityBest practice |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Konrad Hubert Paszkiewicz Audrey eFarbos Paul eO'Neill Karen eMoore |
| spellingShingle |
Konrad Hubert Paszkiewicz Audrey eFarbos Paul eO'Neill Karen eMoore Quality control on the frontier Frontiers in Genetics Sequencing Quality control Illumina contamination Core-facility Best practice |
| author_facet |
Konrad Hubert Paszkiewicz Audrey eFarbos Paul eO'Neill Karen eMoore |
| author_sort |
Konrad Hubert Paszkiewicz |
| title |
Quality control on the frontier |
| title_short |
Quality control on the frontier |
| title_full |
Quality control on the frontier |
| title_fullStr |
Quality control on the frontier |
| title_full_unstemmed |
Quality control on the frontier |
| title_sort |
quality control on the frontier |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Genetics |
| issn |
1664-8021 |
| publishDate |
2014-05-01 |
| description |
In the world of high-throughput sequencing there are numerous challenges to effective data quality control. There are no single quality metrics which are appropriate in all conditions. Here we detail the different open source software used at the Exeter Sequencing Service to provide generic quality control information, as well as more specific metrics for genomic and transcriptomic libraries run on Illumina platforms. |
| topic |
Sequencing Quality control Illumina contamination Core-facility Best practice |
| url |
http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00157/full |
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AT konradhubertpaszkiewicz qualitycontrolonthefrontier AT audreyefarbos qualitycontrolonthefrontier AT pauleoneill qualitycontrolonthefrontier AT karenemoore qualitycontrolonthefrontier |
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