Human serum low molecular mass prostate-specific antigen as biomarker
Background: Prostate-specific antigen (PSA) is a glycoprotein tumor marker known to exist as numerous glycospecies. Investigations on its glycobiochemical properties aimed at their use in the preparation of adjuncts in determining PSA concentration for clinical purposes have accumulated a lot of dat...
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Society of Medical Biochemists of Serbia, Belgrade
2017-01-01
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doaj-407ac7a93b9648dc8a8f5cfbcd5deb842020-11-25T02:38:14ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662017-01-013643223301452-82581704322GHuman serum low molecular mass prostate-specific antigen as biomarkerGoč Sanja0Janković Miroslava1https://orcid.org/0000-0002-7889-5110University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Department for Immunochemistry and Glycobiology, BelgradeUniversity of Belgrade, Institute for the Application of Nuclear Energy, INEP, Department for Immunochemistry and Glycobiology, BelgradeBackground: Prostate-specific antigen (PSA) is a glycoprotein tumor marker known to exist as numerous glycospecies. Investigations on its glycobiochemical properties aimed at their use in the preparation of adjuncts in determining PSA concentration for clinical purposes have accumulated a lot of data on its structural properties. In this study, we reconsidered unexplored ubiquitously present low molecular mass species of PSA regarding to molecular mass, origin and pathophysiological source specificity in order to evaluate them as biomarkers. Methods: Data on low molecular mass PSA-immunoreactive species from sera of subjects with prostate cancer (PCa), benign prostatic hyperplasia (BPH), breast cancer (BCa), and urine of healthy males obtained by on-chip immunoaffinity chromatography combined with mass spectrometry were analyzed. Results: The results obtained indicated PSA species common to BCa, PCa, and BPH at 1 2-1 3 kDa, 1 7-1 9 kDa and 2 1-2 4 kDa. The striking difference in predominant frequencies made the profile characteristic in each examined pathophysiological condition. On the other hand, paired groups of prostatic and extraprostatic PSA contained rare species with small differences among groups concerning individual species. Low molecular mass PSA also included rare species unique for each group of samples. Conclusions: The results obtained revealed that uniformity of low molecular mass PSA-immunoreactive species in sera prevails over diversity related to cancer and non-cancer conditions, but at the same time some of them are molecules with biomarker potential for BPH detection.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2017/1452-82581704322G.pdfbenign prostatic hyperplasiabreast cancerlow molecular mass speciesprostate cancerprostate-specific antigen |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Goč Sanja Janković Miroslava |
spellingShingle |
Goč Sanja Janković Miroslava Human serum low molecular mass prostate-specific antigen as biomarker Journal of Medical Biochemistry benign prostatic hyperplasia breast cancer low molecular mass species prostate cancer prostate-specific antigen |
author_facet |
Goč Sanja Janković Miroslava |
author_sort |
Goč Sanja |
title |
Human serum low molecular mass prostate-specific antigen as biomarker |
title_short |
Human serum low molecular mass prostate-specific antigen as biomarker |
title_full |
Human serum low molecular mass prostate-specific antigen as biomarker |
title_fullStr |
Human serum low molecular mass prostate-specific antigen as biomarker |
title_full_unstemmed |
Human serum low molecular mass prostate-specific antigen as biomarker |
title_sort |
human serum low molecular mass prostate-specific antigen as biomarker |
publisher |
Society of Medical Biochemists of Serbia, Belgrade |
series |
Journal of Medical Biochemistry |
issn |
1452-8258 1452-8266 |
publishDate |
2017-01-01 |
description |
Background: Prostate-specific antigen (PSA) is a glycoprotein tumor marker known to exist as numerous glycospecies. Investigations on its glycobiochemical properties aimed at their use in the preparation of adjuncts in determining PSA concentration for clinical purposes have accumulated a lot of data on its structural properties. In this study, we reconsidered unexplored ubiquitously present low molecular mass species of PSA regarding to molecular mass, origin and pathophysiological source specificity in order to evaluate them as biomarkers. Methods: Data on low molecular mass PSA-immunoreactive species from sera of subjects with prostate cancer (PCa), benign prostatic hyperplasia (BPH), breast cancer (BCa), and urine of healthy males obtained by on-chip immunoaffinity chromatography combined with mass spectrometry were analyzed. Results: The results obtained indicated PSA species common to BCa, PCa, and BPH at 1 2-1 3 kDa, 1 7-1 9 kDa and 2 1-2 4 kDa. The striking difference in predominant frequencies made the profile characteristic in each examined pathophysiological condition. On the other hand, paired groups of prostatic and extraprostatic PSA contained rare species with small differences among groups concerning individual species. Low molecular mass PSA also included rare species unique for each group of samples. Conclusions: The results obtained revealed that uniformity of low molecular mass PSA-immunoreactive species in sera prevails over diversity related to cancer and non-cancer conditions, but at the same time some of them are molecules with biomarker potential for BPH detection. |
topic |
benign prostatic hyperplasia breast cancer low molecular mass species prostate cancer prostate-specific antigen |
url |
https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2017/1452-82581704322G.pdf |
work_keys_str_mv |
AT gocsanja humanserumlowmolecularmassprostatespecificantigenasbiomarker AT jankovicmiroslava humanserumlowmolecularmassprostatespecificantigenasbiomarker |
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