Human serum low molecular mass prostate-specific antigen as biomarker

Background: Prostate-specific antigen (PSA) is a glycoprotein tumor marker known to exist as numerous glycospecies. Investigations on its glycobiochemical properties aimed at their use in the preparation of adjuncts in determining PSA concentration for clinical purposes have accumulated a lot of dat...

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Main Authors: Goč Sanja, Janković Miroslava
Format: Article
Language:English
Published: Society of Medical Biochemists of Serbia, Belgrade 2017-01-01
Series:Journal of Medical Biochemistry
Subjects:
Online Access:https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2017/1452-82581704322G.pdf
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spelling doaj-407ac7a93b9648dc8a8f5cfbcd5deb842020-11-25T02:38:14ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662017-01-013643223301452-82581704322GHuman serum low molecular mass prostate-specific antigen as biomarkerGoč Sanja0Janković Miroslava1https://orcid.org/0000-0002-7889-5110University of Belgrade, Institute for the Application of Nuclear Energy, INEP, Department for Immunochemistry and Glycobiology, BelgradeUniversity of Belgrade, Institute for the Application of Nuclear Energy, INEP, Department for Immunochemistry and Glycobiology, BelgradeBackground: Prostate-specific antigen (PSA) is a glycoprotein tumor marker known to exist as numerous glycospecies. Investigations on its glycobiochemical properties aimed at their use in the preparation of adjuncts in determining PSA concentration for clinical purposes have accumulated a lot of data on its structural properties. In this study, we reconsidered unexplored ubiquitously present low molecular mass species of PSA regarding to molecular mass, origin and pathophysiological source specificity in order to evaluate them as biomarkers. Methods: Data on low molecular mass PSA-immunoreactive species from sera of subjects with prostate cancer (PCa), benign prostatic hyperplasia (BPH), breast cancer (BCa), and urine of healthy males obtained by on-chip immunoaffinity chromatography combined with mass spectrometry were analyzed. Results: The results obtained indicated PSA species common to BCa, PCa, and BPH at 1 2-1 3 kDa, 1 7-1 9 kDa and 2 1-2 4 kDa. The striking difference in predominant frequencies made the profile characteristic in each examined pathophysiological condition. On the other hand, paired groups of prostatic and extraprostatic PSA contained rare species with small differences among groups concerning individual species. Low molecular mass PSA also included rare species unique for each group of samples. Conclusions: The results obtained revealed that uniformity of low molecular mass PSA-immunoreactive species in sera prevails over diversity related to cancer and non-cancer conditions, but at the same time some of them are molecules with biomarker potential for BPH detection.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2017/1452-82581704322G.pdfbenign prostatic hyperplasiabreast cancerlow molecular mass speciesprostate cancerprostate-specific antigen
collection DOAJ
language English
format Article
sources DOAJ
author Goč Sanja
Janković Miroslava
spellingShingle Goč Sanja
Janković Miroslava
Human serum low molecular mass prostate-specific antigen as biomarker
Journal of Medical Biochemistry
benign prostatic hyperplasia
breast cancer
low molecular mass species
prostate cancer
prostate-specific antigen
author_facet Goč Sanja
Janković Miroslava
author_sort Goč Sanja
title Human serum low molecular mass prostate-specific antigen as biomarker
title_short Human serum low molecular mass prostate-specific antigen as biomarker
title_full Human serum low molecular mass prostate-specific antigen as biomarker
title_fullStr Human serum low molecular mass prostate-specific antigen as biomarker
title_full_unstemmed Human serum low molecular mass prostate-specific antigen as biomarker
title_sort human serum low molecular mass prostate-specific antigen as biomarker
publisher Society of Medical Biochemists of Serbia, Belgrade
series Journal of Medical Biochemistry
issn 1452-8258
1452-8266
publishDate 2017-01-01
description Background: Prostate-specific antigen (PSA) is a glycoprotein tumor marker known to exist as numerous glycospecies. Investigations on its glycobiochemical properties aimed at their use in the preparation of adjuncts in determining PSA concentration for clinical purposes have accumulated a lot of data on its structural properties. In this study, we reconsidered unexplored ubiquitously present low molecular mass species of PSA regarding to molecular mass, origin and pathophysiological source specificity in order to evaluate them as biomarkers. Methods: Data on low molecular mass PSA-immunoreactive species from sera of subjects with prostate cancer (PCa), benign prostatic hyperplasia (BPH), breast cancer (BCa), and urine of healthy males obtained by on-chip immunoaffinity chromatography combined with mass spectrometry were analyzed. Results: The results obtained indicated PSA species common to BCa, PCa, and BPH at 1 2-1 3 kDa, 1 7-1 9 kDa and 2 1-2 4 kDa. The striking difference in predominant frequencies made the profile characteristic in each examined pathophysiological condition. On the other hand, paired groups of prostatic and extraprostatic PSA contained rare species with small differences among groups concerning individual species. Low molecular mass PSA also included rare species unique for each group of samples. Conclusions: The results obtained revealed that uniformity of low molecular mass PSA-immunoreactive species in sera prevails over diversity related to cancer and non-cancer conditions, but at the same time some of them are molecules with biomarker potential for BPH detection.
topic benign prostatic hyperplasia
breast cancer
low molecular mass species
prostate cancer
prostate-specific antigen
url https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2017/1452-82581704322G.pdf
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