Single-molecule visualization of conformational changes and substrate transport in the vitamin B12 ABC importer BtuCD-F
The vitamin B12 importer BtuCD-F is a type II ATP-binding cassette transporter. Here the authors use single-molecule fluorescence techniques to analyse ATP hydrolysis and substrate transport in individual BtuCD-F complexes embedded in liposomes, and propose a transport model.
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2017-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-017-01815-7 |