Single-molecule visualization of conformational changes and substrate transport in the vitamin B12 ABC importer BtuCD-F

Single-molecule visualization of conformational changes and substrate transport in the vitamin B12 ABC importer BtuCD-F

The vitamin B12 importer BtuCD-F is a type II ATP-binding cassette transporter. Here the authors use single-molecule fluorescence techniques to analyse ATP hydrolysis and substrate transport in individual BtuCD-F complexes embedded in liposomes, and propose a transport model.

Bibliographic Details
Main Authors: Joris M. H. Goudsmits, Dirk Jan Slotboom, Antoine M. van Oijen
Format: Article
Language:English
Published: Nature Publishing Group 2017-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-01815-7
Description
Summary:The vitamin B12 importer BtuCD-F is a type II ATP-binding cassette transporter. Here the authors use single-molecule fluorescence techniques to analyse ATP hydrolysis and substrate transport in individual BtuCD-F complexes embedded in liposomes, and propose a transport model.
ISSN:2041-1723

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