Estimating the Product Inhibition Constant from Enzyme Kinetic Equations Using the Direct Linear Plot Method in One-Stage Treatment

Estimating the Product Inhibition Constant from Enzyme Kinetic Equations Using the Direct Linear Plot Method in One-Stage Treatment

A direct linear plot was applied to estimate kinetic constants using the product’s competitive inhibition equation. The challenge consisted of estimating three kinetic constants, <i>V<sub>max</sub></i>, <i>K<sub>m</sub></i><sub>,</sub> and...

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Bibliographic Details
Main Authors: Pedro L. Valencia, Bastián Sepúlveda, Diego Gajardo, Carolina Astudillo-Castro
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Catalysts
Subjects:
direct linear plot
median method
product inhibition
kinetic constants
non-parametric
distribution-free method
Online Access:https://www.mdpi.com/2073-4344/10/8/853

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