Low-temperature threshold for egg survival of a post-diapause and non-diapause European aedine strain, <it>Aedes albopictus</it> (Diptera: Culicidae)

• Main Authors: Thomas Stephanie, Obermayr Ulla, Fischer Dominik, Kreyling Juergen, Beierkuhnlein Carl
• Format: Article
• Language: English
• Published: BMC 2012-05-01
• Series: Parasites & Vectors
• Online Access: http://www.parasitesandvectors.com/content/5/1/100

<p>Abstract</p> <p>Background</p> <p>The interplay between global warming and invasive arthropods in temperate zones is of utmost interest in terms of the potential expansions of vector-borne diseases. Up to now, investigations on the recent establishment of mosquito vectors have focused on temperatures during their phases of activity. However, cold temperatures may also act as a strong ecological constraint. Projected changes in winter climate indicate an increase of mean minimum temperatures of the coldest quarter, less frequent days with frost and a shorter frost-season in Europe at the end of the century. Nevertheless, single cold extremes are also expected to persist under warming scenarios, which have a strong impact on reproduction success.</p> <p>Methods</p> <p>Here, the temperature constraints of European <it>Aedes albopictus</it> eggs, which had passed through a diapause, compared to non-diapausing eggs were examined systematically under controlled laboratory conditions. Additionally, one tropical strain of <it>Ae. albopictus</it> and of <it>Ae. aegypti</it> was used in the comparison.</p> <p>Results</p> <p>The lower temperature threshold tolerated by the European eggs of <it>Ae. albopictus</it> which have undergone a diapause, was -10°C for long term exposures (12 and 24h) and -12°C for 1h exposure. Non-diapausing eggs of European <it>Ae. albopictus</it> were found to hatch after a -7°C cold treatment (8, 12 and 24h exposure). Both tropical aedine species only tolerated the long term treatment at -2°C. Neither <it>Ae. albopictus</it> nor <it>Ae. aegypti</it> eggs hatched after being exposed to -15°C. Survival was mainly influenced by temperature (F = 329.2, df = 1, p < 0.001), whereas the duration of the cold treatment only significantly influenced the hatching response at the thermal limits of survival (F = 5.6, df = 1, p = 0.031) but not at 0°C (F = 0.1, df = 1, p = 0.730). Hatching success after the cold treatment was significantly increased in European eggs, which have undergone a diapause compared to non-diapausing eggs (F = 14.7, df = 3, p < 0.001). These results illustrate rapid adaptation.</p> <p>Conclusions</p> <p>Here, low temperature thresholds for aedine mosquito egg survival were detected. The compilation of risk maps for temperate regions can substantially be improved by considering areas where an establishment of a vector population is unlikely due to winter conditions.</p>