Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species

Aim: Wild birds are considered silent vectors of some zoonotic water and food borne pathogens of public health significance. Owing to the importance of Shiga toxin producing Escherichia coli (STEC) as the most pathogenic among the emerging diarrheagenic E. coli groups that can infect man; the presen...

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Main Authors: Hanaa Mohamed Fadel, Rabab Afifi, Dheyazan Mohammed Al-Qabili
Format: Article
Language:English
Published: Veterinary World 2017-09-01
Series:Veterinary World
Subjects:
eae
Online Access:http://www.veterinaryworld.org/Vol.10/September-2017/17.pdf
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spelling doaj-3fe64d6ed1f841759a8e6ea13c6158222021-08-02T08:19:12ZengVeterinary WorldVeterinary World0972-89882231-09162017-09-011091118112810.14202/vetworld.2017.1118-1128Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird speciesHanaa Mohamed Fadel0Rabab Afifi1Dheyazan Mohammed Al-Qabili2Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.Department of Wildlife and Zoo Medicine, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.Department of Veterinary Public Health, Agriculture and Veterinary Medicine College, Thamar University, Yemen.Aim: Wild birds are considered silent vectors of some zoonotic water and food borne pathogens of public health significance. Owing to the importance of Shiga toxin producing Escherichia coli (STEC) as the most pathogenic among the emerging diarrheagenic E. coli groups that can infect man; the present study was designed to detect the occurrence of STEC among wild birds in Egypt. Materials and Methods: A total of 177 intestinal content swab samples originating from five wild bird species were investigated for the presence of E. coli and STEC by standard culture methods. Suspect STEC isolates were further characterized by serotyping, random amplified polymorphic DNA polymerase chain reaction (RAPD PCR), antimicrobial resistance pattern and PCR detection of stx1, stx2, and eae genes. Results: A total of 30 suspect STEC isolates from 30 positive birds' samples were detected and identified on STEC CHROMagar (semi-captive pigeons, 15; house crows, 8; cattle egrets, 3; moorhens, 2; and house teals, 2). 25 isolates were grouped into 13 serogroups (O:20, O:25, O:26, O:27, O:63, O:78, O:111, O:114, O:125, O:128, O:142, O:153, and O:158), while five were rough strains. The distribution of STEC virulence genes among wild birds was as follows: 16 birds carried stx1 gene only (nine pigeons [28.1%], six crows [7.1%], and one cattle egret [5.6%]). stx1 and stx2 genes together were detected in four birds (one cattle egret [5.6%], two moorhens [6.1%], and one house teal, [10%]). Only one pigeon (3.1%) possessed the three alleles. Disk diffusion test results showed that cefixime was the most effective against STEC serotypes with (93.3%) sensitivity, followed by gentamycin (56.7%), and amoxicillin (50%). On the other hand, all the recovered STEC isolates were resistant to cefotaxime, doxycycline, cephalothin, and sulfisoxazole. RAPD fingerprinting using primers OPA-2 and OPA-9 showed that STEC isolates were heterogeneous; they yielded 30 and 27 different clusters, respectively. Conclusion: Wild birds carry STEC and may add to the contamination of the surrounding environment.http://www.veterinaryworld.org/Vol.10/September-2017/17.pdfantibioticeaerandom-amplified polymorphic DNA polymerase chain reactionShiga toxin producing Escherichia colistx1stx2wild birds
collection DOAJ
language English
format Article
sources DOAJ
author Hanaa Mohamed Fadel
Rabab Afifi
Dheyazan Mohammed Al-Qabili
spellingShingle Hanaa Mohamed Fadel
Rabab Afifi
Dheyazan Mohammed Al-Qabili
Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species
Veterinary World
antibiotic
eae
random-amplified polymorphic DNA polymerase chain reaction
Shiga toxin producing Escherichia coli
stx1
stx2
wild birds
author_facet Hanaa Mohamed Fadel
Rabab Afifi
Dheyazan Mohammed Al-Qabili
author_sort Hanaa Mohamed Fadel
title Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species
title_short Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species
title_full Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species
title_fullStr Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species
title_full_unstemmed Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species
title_sort characterization and zoonotic impact of shiga toxin producing escherichia coli in some wild bird species
publisher Veterinary World
series Veterinary World
issn 0972-8988
2231-0916
publishDate 2017-09-01
description Aim: Wild birds are considered silent vectors of some zoonotic water and food borne pathogens of public health significance. Owing to the importance of Shiga toxin producing Escherichia coli (STEC) as the most pathogenic among the emerging diarrheagenic E. coli groups that can infect man; the present study was designed to detect the occurrence of STEC among wild birds in Egypt. Materials and Methods: A total of 177 intestinal content swab samples originating from five wild bird species were investigated for the presence of E. coli and STEC by standard culture methods. Suspect STEC isolates were further characterized by serotyping, random amplified polymorphic DNA polymerase chain reaction (RAPD PCR), antimicrobial resistance pattern and PCR detection of stx1, stx2, and eae genes. Results: A total of 30 suspect STEC isolates from 30 positive birds' samples were detected and identified on STEC CHROMagar (semi-captive pigeons, 15; house crows, 8; cattle egrets, 3; moorhens, 2; and house teals, 2). 25 isolates were grouped into 13 serogroups (O:20, O:25, O:26, O:27, O:63, O:78, O:111, O:114, O:125, O:128, O:142, O:153, and O:158), while five were rough strains. The distribution of STEC virulence genes among wild birds was as follows: 16 birds carried stx1 gene only (nine pigeons [28.1%], six crows [7.1%], and one cattle egret [5.6%]). stx1 and stx2 genes together were detected in four birds (one cattle egret [5.6%], two moorhens [6.1%], and one house teal, [10%]). Only one pigeon (3.1%) possessed the three alleles. Disk diffusion test results showed that cefixime was the most effective against STEC serotypes with (93.3%) sensitivity, followed by gentamycin (56.7%), and amoxicillin (50%). On the other hand, all the recovered STEC isolates were resistant to cefotaxime, doxycycline, cephalothin, and sulfisoxazole. RAPD fingerprinting using primers OPA-2 and OPA-9 showed that STEC isolates were heterogeneous; they yielded 30 and 27 different clusters, respectively. Conclusion: Wild birds carry STEC and may add to the contamination of the surrounding environment.
topic antibiotic
eae
random-amplified polymorphic DNA polymerase chain reaction
Shiga toxin producing Escherichia coli
stx1
stx2
wild birds
url http://www.veterinaryworld.org/Vol.10/September-2017/17.pdf
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