Lacrimal gland primary acinar cell culture: the role of insulin

• Main Authors: Leonardo Tannus Malki, Ana Carolina Dias, Angelica Gobbi Jorge, Carolina Maria Módulo, Eduardo Melani Rocha
• Format: Article
• Language: English
• Published: Conselho Brasileiro de Oftalmologia 2016-04-01
• Series: Arquivos Brasileiros de Oftalmologia
• Subjects: Células acinares; Glândula lacrimal; Aparelho lacrimal; Insulina; Peroxidase; Contagem de células; Medicina regenerativa; Engenharia tecidual; Animais; Ratos Wistar
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492016000200105&lng=en&tlng=en

ABSTRACT Purpose: The goal of the present study was to establish a protocol for primary culture of lacrimal gland acinar cells (LGACs) and to assess the effect of adding insulin to the culture media. Methods: LGACs were isolated and cultured from lacrimal glands of Wistar male rats. The study outcomes included cell number, viability, and peroxidase release over time and in response to three concentrations of insulin (0.5, 5.0, and 50.0 μg/mL). Results: In LGAC primary culture, cells started to form clusters by day 3. There was a time-response pattern of peroxidase release, which rose by day 6, in response to carbachol. Culture viability lasted for 12 days. An insulin concentration of 5.0 μg/mL in the culture medium resulted in higher viability and secretory capacity. Conclusions: The present method simplifies the isolation and culture of LGACs. The data confirmed the relevance of adding insulin to maintain LGACs in culture.