Identification of proteins in fluid collected from nerve regeneration chambers

Identification of proteins in fluid collected from nerve regeneration chambers

We examined whether there are novel neurotrophic factors (NTFs) in nerve regeneration conditioned fluid (NRCF). Nerve regeneration chamber models were established in the sciatic nerves of 25 New Zealand rabbits, and NRCF was extracted from the chambers l week postoperatively. Proteins in NR...

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Bibliographic Details
Main Authors: Ye Yilin, Li Li, Li Qingfeng
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2014-01-01
Series:Archives of Biological Sciences
Subjects:
Nerve regeneration conditioned fluid
neurotrophic factor
protein extraction
rabbit
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641403035Y.pdf
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spelling doaj-3f470ace14894b118ac56a561551f3452020-11-24T20:48:05ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392014-01-016631035103910.2298/ABS1403035Y0354-46641403035YIdentification of proteins in fluid collected from nerve regeneration chambersYe Yilin0Li Li1Li Qingfeng2Anhui University of Traditional Chinese Medicine, Department of Medical Cosmetology, Anhui Province, ChinaAnhui University of Traditional Chinese Medicine, Department of Pharmacology, Anhui Province, ChinaAffiliated Ninth People’s Hospital of Shanghai Jiao Tong University, Department of Plastic Surgery, Shanghai, ChinaWe examined whether there are novel neurotrophic factors (NTFs) in nerve regeneration conditioned fluid (NRCF). Nerve regeneration chamber models were established in the sciatic nerves of 25 New Zealand rabbits, and NRCF was extracted from the chambers l week postoperatively. Proteins in NRCF were separated by native polyacrylamide gel electrophoresis (PAGE), and Western blot and ELISA were used to identify the proteins. A novel NTF was identified in a protein fraction corresponding to 220 kDa.http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641403035Y.pdfNerve regeneration conditioned fluidneurotrophic factorprotein extractionrabbit
collection DOAJ
language English
format Article
sources DOAJ
author Ye Yilin
Li Li
Li Qingfeng
spellingShingle Ye Yilin
Li Li
Li Qingfeng
Identification of proteins in fluid collected from nerve regeneration chambers
Archives of Biological Sciences
Nerve regeneration conditioned fluid
neurotrophic factor
protein extraction
rabbit
author_facet Ye Yilin
Li Li
Li Qingfeng
author_sort Ye Yilin
title Identification of proteins in fluid collected from nerve regeneration chambers
title_short Identification of proteins in fluid collected from nerve regeneration chambers
title_full Identification of proteins in fluid collected from nerve regeneration chambers
title_fullStr Identification of proteins in fluid collected from nerve regeneration chambers
title_full_unstemmed Identification of proteins in fluid collected from nerve regeneration chambers
title_sort identification of proteins in fluid collected from nerve regeneration chambers
publisher University of Belgrade, University of Novi Sad
series Archives of Biological Sciences
issn 0354-4664
1821-4339
publishDate 2014-01-01
description We examined whether there are novel neurotrophic factors (NTFs) in nerve regeneration conditioned fluid (NRCF). Nerve regeneration chamber models were established in the sciatic nerves of 25 New Zealand rabbits, and NRCF was extracted from the chambers l week postoperatively. Proteins in NRCF were separated by native polyacrylamide gel electrophoresis (PAGE), and Western blot and ELISA were used to identify the proteins. A novel NTF was identified in a protein fraction corresponding to 220 kDa.
topic Nerve regeneration conditioned fluid
neurotrophic factor
protein extraction
rabbit
url http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641403035Y.pdf
work_keys_str_mv AT yeyilin identificationofproteinsinfluidcollectedfromnerveregenerationchambers
AT lili identificationofproteinsinfluidcollectedfromnerveregenerationchambers
AT liqingfeng identificationofproteinsinfluidcollectedfromnerveregenerationchambers
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