Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques

Abstract While myelin deficit of the central nervous system leads to several severe diseases, the definitive diagnostic means are lacking. We proposed and performed terahertz time-domain spectroscopy (THz-TDS) combined with chemometric techniques to discriminate and evaluate the severity of myelin d...

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Main Authors:	Yi Zou, Jiang Li, Yiyuan Cui, Peiren Tang, Lianghui Du, Tunan Chen, Kun Meng, Qiao Liu, Hua Feng, Jianheng Zhao, Mina Chen, Li-Guo Zhu
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-07-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-017-05554-z

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spelling	doaj-3f3d22f40e51404fab398bb26bb0e4902020-12-08T01:43:10ZengNature Publishing GroupScientific Reports2045-23222017-07-01711910.1038/s41598-017-05554-zTerahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric TechniquesYi Zou0Jiang Li1Yiyuan Cui2Peiren Tang3Lianghui Du4Tunan Chen5Kun Meng6Qiao Liu7Hua Feng8Jianheng Zhao9Mina Chen10Li-Guo Zhu11Interdisciplinary Laboratory of Physics and Biomedicine, Institute of Fluid Physics, China Academy of Engineering PhysicsInterdisciplinary Laboratory of Physics and Biomedicine, Institute of Fluid Physics, China Academy of Engineering PhysicsThe State Key Laboratory of Biotherapy, West China Hospital, Sichuan UniversityInterdisciplinary Laboratory of Physics and Biomedicine, Institute of Fluid Physics, China Academy of Engineering PhysicsInterdisciplinary Laboratory of Physics and Biomedicine, Institute of Fluid Physics, China Academy of Engineering PhysicsDepartment of Neurosurgery, Southwest Hospital, Third Military Medical UniversityInterdisciplinary Laboratory of Physics and Biomedicine, Institute of Fluid Physics, China Academy of Engineering PhysicsInterdisciplinary Laboratory of Physics and Biomedicine, Institute of Fluid Physics, China Academy of Engineering PhysicsDepartment of Neurosurgery, Southwest Hospital, Third Military Medical UniversityInterdisciplinary Laboratory of Physics and Biomedicine, Institute of Fluid Physics, China Academy of Engineering PhysicsThe State Key Laboratory of Biotherapy, West China Hospital, Sichuan UniversityInterdisciplinary Laboratory of Physics and Biomedicine, Institute of Fluid Physics, China Academy of Engineering PhysicsAbstract While myelin deficit of the central nervous system leads to several severe diseases, the definitive diagnostic means are lacking. We proposed and performed terahertz time-domain spectroscopy (THz-TDS) combined with chemometric techniques to discriminate and evaluate the severity of myelin deficit in mouse and rhesus monkey brains. The THz refractive index and absorption coefficient of paraffin-embedded brain tissues from both normal and mutant dysmyelinating mice are shown. Principal component analysis of time-domain THz signal (PCA-tdTHz) and absorption-refractive index relation of THz spectrum identified myelin deficit without exogenous labeling or any pretreatment. Further, with the established PCA-tdTHz, we evaluated the severity of myelin deficit lesions in rhesus monkey brain induced by experimental autoimmune encephalomyelitis, which is the most-studied animal model of multiple sclerosis. The results well matched the pathological analysis, indicating that PCA-tdTHz is a quick, powerful, evolving tool for identification and evaluation myelin deficit in preclinical animals and potentially in para-clinical human biopsy.https://doi.org/10.1038/s41598-017-05554-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yi Zou Jiang Li Yiyuan Cui Peiren Tang Lianghui Du Tunan Chen Kun Meng Qiao Liu Hua Feng Jianheng Zhao Mina Chen Li-Guo Zhu
spellingShingle	Yi Zou Jiang Li Yiyuan Cui Peiren Tang Lianghui Du Tunan Chen Kun Meng Qiao Liu Hua Feng Jianheng Zhao Mina Chen Li-Guo Zhu Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques Scientific Reports
author_facet	Yi Zou Jiang Li Yiyuan Cui Peiren Tang Lianghui Du Tunan Chen Kun Meng Qiao Liu Hua Feng Jianheng Zhao Mina Chen Li-Guo Zhu
author_sort	Yi Zou
title	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_short	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_full	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_fullStr	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_full_unstemmed	Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques
title_sort	terahertz spectroscopic diagnosis of myelin deficit brain in mice and rhesus monkey with chemometric techniques
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2017-07-01
description	Abstract While myelin deficit of the central nervous system leads to several severe diseases, the definitive diagnostic means are lacking. We proposed and performed terahertz time-domain spectroscopy (THz-TDS) combined with chemometric techniques to discriminate and evaluate the severity of myelin deficit in mouse and rhesus monkey brains. The THz refractive index and absorption coefficient of paraffin-embedded brain tissues from both normal and mutant dysmyelinating mice are shown. Principal component analysis of time-domain THz signal (PCA-tdTHz) and absorption-refractive index relation of THz spectrum identified myelin deficit without exogenous labeling or any pretreatment. Further, with the established PCA-tdTHz, we evaluated the severity of myelin deficit lesions in rhesus monkey brain induced by experimental autoimmune encephalomyelitis, which is the most-studied animal model of multiple sclerosis. The results well matched the pathological analysis, indicating that PCA-tdTHz is a quick, powerful, evolving tool for identification and evaluation myelin deficit in preclinical animals and potentially in para-clinical human biopsy.
url	https://doi.org/10.1038/s41598-017-05554-z
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Terahertz Spectroscopic Diagnosis of Myelin Deficit Brain in Mice and Rhesus Monkey with Chemometric Techniques

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