Optimisation of automated ribosomal intergenic spacer analysis for the estimation of microbial diversity in fynbos soil

Optimisation of automated ribosomal intergenic spacer analysis for the estimation of microbial diversity in fynbos soil

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Automated ribosomal intergenic spacer analysis (ARISA) has become a commonly used molecular technique for the study of microbial populations in environmental samples. The reproducibility and accuracy of ARISA, with and without the polymerase chain reaction (PCR) are important aspects that influence...

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Bibliographic Details
Main Authors: Etienne Slabbert, Carel J. van Heerden, Karin Jacobs
Format: Article
Language:English
Published: Academy of Science of South Africa 2010-08-01
Series:South African Journal of Science
Subjects:
automated ribosomal intergenic spacer analysis
binning
fungal ecology
fynbos fungi
molecular community fingerprinting
soil fungi
Online Access:http://archive.sajs.co.za/index.php/SAJS/article/view/329

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