Bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential [version 2; peer review: 1 approved, 2 approved with reservations]

Background: Bio-electrospray (BES) is a jet-based delivery system driven by an electric field that has the ability to form micro to nano-sized droplets. It holds great potential as a tissue engineering tool as it can be used to place cells into specific patterns. As the human central nervous system...

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Main Authors: Citlali Helenes González, Suwan N. Jayasinghe, Patrizia Ferretti
Format: Article
Language:English
Published: F1000 Research Ltd 2020-07-01
Series:F1000Research
Online Access:https://f1000research.com/articles/9-267/v2
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spelling doaj-3f2a06c881f94e1590717d04eed01bb22020-11-25T02:53:11ZengF1000 Research LtdF1000Research2046-14022020-07-01910.12688/f1000research.19901.227411Bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential [version 2; peer review: 1 approved, 2 approved with reservations]Citlali Helenes González0Suwan N. Jayasinghe1Patrizia Ferretti2Stem Cell and Regenerative Medicine Section, UCL Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UKBioPhysics Group, Department of Mechanical Engineering, University College London, London, WC1E 7JE, UKStem Cell and Regenerative Medicine Section, UCL Great Ormond Street Institute of Child Health, University College London, London, WC1N 1EH, UKBackground: Bio-electrospray (BES) is a jet-based delivery system driven by an electric field that has the ability to form micro to nano-sized droplets. It holds great potential as a tissue engineering tool as it can be used to place cells into specific patterns. As the human central nervous system (CNS) cannot be studied in vivo at the cellular and molecular level, in vitro CNS models are needed. Human neural stem cells (hNSCs) are the CNS building block as they can generate both neurones and glial cells. Methods: Here we assessed for the first time how hNSCs respond to BES. To this purpose, different hNSC lines were sprayed at 10 kV and their ability to survive, grow and differentiate was assessed at different time points. Results: BES induced only a small and transient decrease in hNSC metabolic activity, from which the cells recovered by day 6, and no significant increase in cell death was observed, as assessed by flow cytometry. Furthermore, bio-electrosprayed hNSCs differentiated as efficiently as controls into neurones, astrocytes and oligodendrocytes, as shown by morphological, protein and gene expression analysis. Conclusions: This study highlights the robustness of hNSCs and identifies BES as a suitable technology that could be developed for the direct deposition of these cells in specific locations and configurations.https://f1000research.com/articles/9-267/v2
collection DOAJ
language English
format Article
sources DOAJ
author Citlali Helenes González
Suwan N. Jayasinghe
Patrizia Ferretti
spellingShingle Citlali Helenes González
Suwan N. Jayasinghe
Patrizia Ferretti
Bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential [version 2; peer review: 1 approved, 2 approved with reservations]
F1000Research
author_facet Citlali Helenes González
Suwan N. Jayasinghe
Patrizia Ferretti
author_sort Citlali Helenes González
title Bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential [version 2; peer review: 1 approved, 2 approved with reservations]
title_short Bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential [version 2; peer review: 1 approved, 2 approved with reservations]
title_full Bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential [version 2; peer review: 1 approved, 2 approved with reservations]
title_fullStr Bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential [version 2; peer review: 1 approved, 2 approved with reservations]
title_full_unstemmed Bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential [version 2; peer review: 1 approved, 2 approved with reservations]
title_sort bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential [version 2; peer review: 1 approved, 2 approved with reservations]
publisher F1000 Research Ltd
series F1000Research
issn 2046-1402
publishDate 2020-07-01
description Background: Bio-electrospray (BES) is a jet-based delivery system driven by an electric field that has the ability to form micro to nano-sized droplets. It holds great potential as a tissue engineering tool as it can be used to place cells into specific patterns. As the human central nervous system (CNS) cannot be studied in vivo at the cellular and molecular level, in vitro CNS models are needed. Human neural stem cells (hNSCs) are the CNS building block as they can generate both neurones and glial cells. Methods: Here we assessed for the first time how hNSCs respond to BES. To this purpose, different hNSC lines were sprayed at 10 kV and their ability to survive, grow and differentiate was assessed at different time points. Results: BES induced only a small and transient decrease in hNSC metabolic activity, from which the cells recovered by day 6, and no significant increase in cell death was observed, as assessed by flow cytometry. Furthermore, bio-electrosprayed hNSCs differentiated as efficiently as controls into neurones, astrocytes and oligodendrocytes, as shown by morphological, protein and gene expression analysis. Conclusions: This study highlights the robustness of hNSCs and identifies BES as a suitable technology that could be developed for the direct deposition of these cells in specific locations and configurations.
url https://f1000research.com/articles/9-267/v2
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AT suwannjayasinghe bioelectrosprayedhumanneuralstemcellsareviableandmaintaintheirdifferentiationpotentialversion2peerreview1approved2approvedwithreservations
AT patriziaferretti bioelectrosprayedhumanneuralstemcellsareviableandmaintaintheirdifferentiationpotentialversion2peerreview1approved2approvedwithreservations
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