Acidic pH Decreases the Endonuclease Activity of Initiator RepB and Increases the Stability of the Covalent RepB-DNA Intermediate while Has Only a Limited Effect on the Replication of Plasmid pMV158 in Lactococcus lactis

Acidic pH Decreases the Endonuclease Activity of Initiator RepB and Increases the Stability of the Covalent RepB-DNA Intermediate while Has Only a Limited Effect on the Replication of Plasmid pMV158 in Lactococcus lactis

Plasmid vectors constitute a valuable tool for homologous and heterologous gene expression, for characterization of promoter and regulatory regions, and for genetic manipulation and labeling of bacteria. During the last years, a series of vectors based on promiscuous replicons of the pMV158 family h...

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Bibliographic Details
Main Authors: Rafael Valdelvira, Lorena Bordanaba-Ruiseco, Cristina Martín-Huestamendía, José Angel Ruiz-Masó, Gloria del Solar
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Molecular Biosciences
Subjects:
rolling circle replication (RCR)
promiscuous plasmid pMV158
nucleophilic attack
endonuclease activity
strand-transfer activity
covalent RepB-DNA adduct
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2021.634461/full

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