A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
Constructing biosynthetic pathways to study and engineer glycoprotein structures is difficult. Here, the authors use GlycoPRIME, a cell-free workflow for mixing-and-matching glycosylation enzymes, to evaluate 37 putative glycosylation pathways and discover routes to 18 new glycoprotein structures
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2019-11-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-12024-9 |