A cell-free biosynthesis platform for modular construction of protein glycosylation pathways
Constructing biosynthetic pathways to study and engineer glycoprotein structures is difficult. Here, the authors use GlycoPRIME, a cell-free workflow for mixing-and-matching glycosylation enzymes, to evaluate 37 putative glycosylation pathways and discover routes to 18 new glycoprotein structures
Bibliographic Details
| Main Authors: |
Weston Kightlinger,
Katherine E. Duncker,
Ashvita Ramesh,
Ariel H. Thames,
Aravind Natarajan,
Jessica C. Stark,
Allen Yang,
Liang Lin,
Milan Mrksich,
Matthew P. DeLisa,
Michael C. Jewett |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Publishing Group
2019-11-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-019-12024-9
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