Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology

Lipase is a lipids hydrolyze enzyme which are widely used in various industries such as chemical, pharmaceutical, food industries, and detergents. Bacillus licheniformis F11.4 is one of the bacteria with potential source of lipase. This study aimed to obtain optimum production of lipase from B. lic...

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Format: Article
Language:English
Published: Indonesian Society for Microbiology 2017-06-01
Series:Microbiology Indonesia
Subjects:
Online Access:https://jurnal.permi.or.id/index.php/mionline/article/view/532
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spelling doaj-3eb11ead6b83461c85f55ba1dbe067a12021-08-20T12:47:47ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752017-06-0110410.5454/mi.10.4.4Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology Lipase is a lipids hydrolyze enzyme which are widely used in various industries such as chemical, pharmaceutical, food industries, and detergents. Bacillus licheniformis F11.4 is one of the bacteria with potential source of lipase. This study aimed to obtain optimum production of lipase from B. licheniformis F11.4 by optimizing the composition of media and pH values with fish flour as a replacement for peptone and yeast extract based medium. Selection of the significant factors used a 2-level factorial design. The upper limit and lower limit of the selected factors was optimized using Central Composite Design (CCD) and the data analysis was performed using the Response Surface Methodology (RSM). Fermentation was carried out in erlenmeyer at initial pH 8 and a temperature of 37 °C, using a shaker incubator at 150 rpm. A fermentation system for lipases production is considered optimal when its desirability value closes to 1. By using numerical optimization, an optimal medium could be obtained, i.e. consisting of OO:CPO 0.14 % (w/v) and fish flour 2% (w/v), at pH 8 and 150 rpm, which produced lipase with enzyme activity of 1.563 U mL-1 and protein level of 0.08 mg mL-1.Furthermore, the results are verified in the Erlenmeyer, working volume of 50 mL, pH = 8, T = 37 °C, agitation 150 rpm, t=18 hours, the activity of lipase and protein levels are 1.568 ± 0.014 U mL-1 and 0.072 ± 0.006 mg mL-1 respectively.The results showed that the optimum condition lipase activity was 1.568 U mL-1 so that the increase in the activity of only 75% compared to before optimization. https://jurnal.permi.or.id/index.php/mionline/article/view/532Bacillus licheniformis F11.4Central Composite Designenzyme activityprotein contentResponse Surface Methodology
collection DOAJ
language English
format Article
sources DOAJ
title Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology
spellingShingle Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology
Microbiology Indonesia
Bacillus licheniformis F11.4
Central Composite Design
enzyme activity
protein content
Response Surface Methodology
title_short Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology
title_full Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology
title_fullStr Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology
title_full_unstemmed Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology
title_sort optimization of lipases production bybacillus licheniformis f11.4 using response surface methodology
publisher Indonesian Society for Microbiology
series Microbiology Indonesia
issn 1978-3477
2087-8575
publishDate 2017-06-01
description Lipase is a lipids hydrolyze enzyme which are widely used in various industries such as chemical, pharmaceutical, food industries, and detergents. Bacillus licheniformis F11.4 is one of the bacteria with potential source of lipase. This study aimed to obtain optimum production of lipase from B. licheniformis F11.4 by optimizing the composition of media and pH values with fish flour as a replacement for peptone and yeast extract based medium. Selection of the significant factors used a 2-level factorial design. The upper limit and lower limit of the selected factors was optimized using Central Composite Design (CCD) and the data analysis was performed using the Response Surface Methodology (RSM). Fermentation was carried out in erlenmeyer at initial pH 8 and a temperature of 37 °C, using a shaker incubator at 150 rpm. A fermentation system for lipases production is considered optimal when its desirability value closes to 1. By using numerical optimization, an optimal medium could be obtained, i.e. consisting of OO:CPO 0.14 % (w/v) and fish flour 2% (w/v), at pH 8 and 150 rpm, which produced lipase with enzyme activity of 1.563 U mL-1 and protein level of 0.08 mg mL-1.Furthermore, the results are verified in the Erlenmeyer, working volume of 50 mL, pH = 8, T = 37 °C, agitation 150 rpm, t=18 hours, the activity of lipase and protein levels are 1.568 ± 0.014 U mL-1 and 0.072 ± 0.006 mg mL-1 respectively.The results showed that the optimum condition lipase activity was 1.568 U mL-1 so that the increase in the activity of only 75% compared to before optimization.
topic Bacillus licheniformis F11.4
Central Composite Design
enzyme activity
protein content
Response Surface Methodology
url https://jurnal.permi.or.id/index.php/mionline/article/view/532
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