Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing

Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing

Here, we report a new homogeneous DNA amplification-based aptamer assay for small analyte sensing. The aptamer of adenosine chosen as the model analyte was split into two fragments able to assemble in the presence of target. Primers were introduced at extremities of one fragment in order to generate...

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Bibliographic Details
Main Authors: Emmanuelle eFiore, Eric eDausse, Hervé eDubouchaud, Eric ePeyrin, Corinne eRavelet
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-08-01
Series:Frontiers in Chemistry
Subjects:
Aptamers, Nucleotide
Biosensors
capillary electrophoresis
Fluorescence detection
PCR amplification
Online Access:http://journal.frontiersin.org/Journal/10.3389/fchem.2015.00049/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fchem.2015.00049/full

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