Ekspresi Rekombinan Gen Protein Selubung Pepper vein yellows virus

• Main Authors: Rita Kurnia Apindiati, Gede Suastika, Kikin Hamzah Mutaqin
• Format: Article
• Language: Indonesian
• Published: Perhimpunan Fitopatologi Indonesia 2015-09-01
• Series: Jurnal Fitopatologi Indonesia
• Subjects: antiserum, expression vector, recombinant, SDS-PAGE
• Online Access: http://journal.ipb.ac.id/index.php/jfiti/article/view/10101

<p><em>Pepper vein yellows virus </em>(PeVYV) isolate from Bali have been identified from pepper plants with chlorosis symptoms. Specific antiserum of PeVYV had not available yet commercially. One of the advance techniques in providing a source of abundant antigen for antiserum production is through molecular approach by overexpressed the coat protein gene in suitable bacterial expression system. PeVYV coat protein gene of ~650 bp in size was amplified using specific primers, then was cloned into pQE30 expression vector and was over expressed in <em>E. coli </em>strain M15 [pREP4]. SDS-PAGE analysis showed that the recombinant coat protein gene of PeVYV was successfully expressed protein band with size of ~25 kDa at 6 hours after induction by 0.5 mM IPTG on 37 °C.</p>