Comparative Study of the Effect of 5,6-Dihydroergosterol and 3-<i>epi</i>-5,6-dihydroergosterol on Chemokine Expression in Human Keratinocytes
5,6-Dihydroergosterol-glucose is an organic synthetic derivative of spinasterol-glucose, which has potent anti-inflammatory activity. We previously synthesized alpha and beta anomers of DHE-glycosides and compared their inhibitory activity on CCL17 and CCL22 mRNA expression induced by TNF-α...
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doaj-3e78281d3adf44d1a5557f1bfaf2a3f12020-11-25T02:03:24ZengMDPI AGMolecules1420-30492020-01-0125352210.3390/molecules25030522molecules25030522Comparative Study of the Effect of 5,6-Dihydroergosterol and 3-<i>epi</i>-5,6-dihydroergosterol on Chemokine Expression in Human KeratinocytesYe Seul Park0Hye Jin Moon1Kwang Hyun Ahn2Tae Hoon Lee3Hakwon Kim4Department of Applied Chemistry and Global Center for Pharmaceutical Ingredient Materials, Kyung Hee Uinveristy, Yongin-si, Gyeonggi-do 17104, KoreaDepartment of Applied Chemistry and Global Center for Pharmaceutical Ingredient Materials, Kyung Hee Uinveristy, Yongin-si, Gyeonggi-do 17104, KoreaDepartment of Applied Chemistry and Global Center for Pharmaceutical Ingredient Materials, Kyung Hee Uinveristy, Yongin-si, Gyeonggi-do 17104, KoreaDepartment of Applied Chemistry and Global Center for Pharmaceutical Ingredient Materials, Kyung Hee Uinveristy, Yongin-si, Gyeonggi-do 17104, KoreaDepartment of Applied Chemistry and Global Center for Pharmaceutical Ingredient Materials, Kyung Hee Uinveristy, Yongin-si, Gyeonggi-do 17104, Korea5,6-Dihydroergosterol-glucose is an organic synthetic derivative of spinasterol-glucose, which has potent anti-inflammatory activity. We previously synthesized alpha and beta anomers of DHE-glycosides and compared their inhibitory activity on CCL17 and CCL22 mRNA expression induced by TNF-α/IFN-γ in activated HaCaTs. Recently, we synthesized a new type of DHE-glycosides, 3-<i>epi</i>-5,6-dihydroergosterol(3-<i>epi</i>-DHE)-glycosides, and compared its inhibitory activity on mRNA expression levels of CCL17 and CCL22 in TNF-α/IFN-γ-induced HaCaT. DHE-Xly did not affect TNF-α/IFN-γ induced CCL17 and CCL22 mRNA expression in HaCaTs, however, 3-<i>epi</i>-DHE-Xly strongly inhibited TNF-α/IFN-γ induced CCL17 and CCL22 mRNA expression levels in human keratinocytes. These results provide important clues for development of chronic dermatitis treatment via inhibition of chemokine expression using DHE derivatives.https://www.mdpi.com/1420-3049/25/3/522anti-inflammationccl17/tarcccl22/mdc3-<i>epi</i>-5,6-dihydroergosterol glycosidechronic dermatitis treatment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ye Seul Park Hye Jin Moon Kwang Hyun Ahn Tae Hoon Lee Hakwon Kim |
spellingShingle |
Ye Seul Park Hye Jin Moon Kwang Hyun Ahn Tae Hoon Lee Hakwon Kim Comparative Study of the Effect of 5,6-Dihydroergosterol and 3-<i>epi</i>-5,6-dihydroergosterol on Chemokine Expression in Human Keratinocytes Molecules anti-inflammation ccl17/tarc ccl22/mdc 3-<i>epi</i>-5,6-dihydroergosterol glycoside chronic dermatitis treatment |
author_facet |
Ye Seul Park Hye Jin Moon Kwang Hyun Ahn Tae Hoon Lee Hakwon Kim |
author_sort |
Ye Seul Park |
title |
Comparative Study of the Effect of 5,6-Dihydroergosterol and 3-<i>epi</i>-5,6-dihydroergosterol on Chemokine Expression in Human Keratinocytes |
title_short |
Comparative Study of the Effect of 5,6-Dihydroergosterol and 3-<i>epi</i>-5,6-dihydroergosterol on Chemokine Expression in Human Keratinocytes |
title_full |
Comparative Study of the Effect of 5,6-Dihydroergosterol and 3-<i>epi</i>-5,6-dihydroergosterol on Chemokine Expression in Human Keratinocytes |
title_fullStr |
Comparative Study of the Effect of 5,6-Dihydroergosterol and 3-<i>epi</i>-5,6-dihydroergosterol on Chemokine Expression in Human Keratinocytes |
title_full_unstemmed |
Comparative Study of the Effect of 5,6-Dihydroergosterol and 3-<i>epi</i>-5,6-dihydroergosterol on Chemokine Expression in Human Keratinocytes |
title_sort |
comparative study of the effect of 5,6-dihydroergosterol and 3-<i>epi</i>-5,6-dihydroergosterol on chemokine expression in human keratinocytes |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-01-01 |
description |
5,6-Dihydroergosterol-glucose is an organic synthetic derivative of spinasterol-glucose, which has potent anti-inflammatory activity. We previously synthesized alpha and beta anomers of DHE-glycosides and compared their inhibitory activity on CCL17 and CCL22 mRNA expression induced by TNF-α/IFN-γ in activated HaCaTs. Recently, we synthesized a new type of DHE-glycosides, 3-<i>epi</i>-5,6-dihydroergosterol(3-<i>epi</i>-DHE)-glycosides, and compared its inhibitory activity on mRNA expression levels of CCL17 and CCL22 in TNF-α/IFN-γ-induced HaCaT. DHE-Xly did not affect TNF-α/IFN-γ induced CCL17 and CCL22 mRNA expression in HaCaTs, however, 3-<i>epi</i>-DHE-Xly strongly inhibited TNF-α/IFN-γ induced CCL17 and CCL22 mRNA expression levels in human keratinocytes. These results provide important clues for development of chronic dermatitis treatment via inhibition of chemokine expression using DHE derivatives. |
topic |
anti-inflammation ccl17/tarc ccl22/mdc 3-<i>epi</i>-5,6-dihydroergosterol glycoside chronic dermatitis treatment |
url |
https://www.mdpi.com/1420-3049/25/3/522 |
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