ISOLATION AND DETERMINATION OF KEMBANG SEPATU FLOWERS (Hibiscus rosa-sinensis L.) ETHANOLIC EXTRACT USING SPECTRODENSITOMETRY

• Main Authors: Mimiek Murrukmihadi, Kurniati Pamungkas
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada 2015-11-01
• Series: Majalah Obat Tradisional
• Subjects: Bunga kembang sepatu, isolasi senyawa, penetapan kadar, alkaloid, spektrodensitometri.
• Online Access: https://jurnal.ugm.ac.id/TradMedJ/article/view/8081
• ISSN: 1410-5918; 2406-9086

This research aims to isolate and determine relative concentration of alkaloid in Hibiscus rosa-sinensis L flowers. using spectrodensitometry. Ethanolic extract of H. rosa-sinensis flowers was made by maceration methode using etanol 70% as solvent. Fractionation of alkaloid compound was using VLC (Vaccum Liquid Chromatography) and using organic solfent mixture of methanol-ethyl acetate in variation concentration. Isolate was used as standard curve, then the concentration of H. rosa-sinensis flowers extract was determined using spectrodensitometry with silica gel as stationer phase and ethyl acetate: methanol ( 1:5 v / v) as mobile phase. The relative concentration of alkaloid was calculated by using standard curve Y = 29023.75 X + 5809.35, r = 0.996. Determination of isolate showed the relative concentration of alkaloid was 0.210 ± 0.035 % and this can be used as marker compound.