Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In Vivo

The generation of mature, functional, thyroid follicular cells from pluripotent stem cells would potentially provide a therapeutic benefit for patients with hypothyroidism, but in vitro differentiation remains difficult. We earlier reported the in vivo generation of lung organs via blastocyst comple...

Full description

Bibliographic Details
Main Authors: Qingsong Ran, Qiliang Zhou, Kanako Oda, Akihiro Yasue, Manabu Abe, Xulu Ye, Yingchun Li, Toshikuni Sasaoka, Kenji Sakimura, Yoichi Ajioka, Yasuo Saijo
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Endocrinology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fendo.2020.609697/full
id doaj-3e63c86498fa41d9af0175cc5f7db864
record_format Article
spelling doaj-3e63c86498fa41d9af0175cc5f7db8642020-12-14T05:45:32ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922020-12-011110.3389/fendo.2020.609697609697Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In VivoQingsong Ran0Qiliang Zhou1Kanako Oda2Akihiro Yasue3Manabu Abe4Xulu Ye5Yingchun Li6Toshikuni Sasaoka7Kenji Sakimura8Yoichi Ajioka9Yasuo Saijo10Department of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, JapanDepartment of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, JapanDepartment of Comparative and Experimental Medicine, Brain Research Institute, Niigata University, Niigata, JapanDepartment of Orthodontics and Dentofacial Orthopedics, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Animal Model Development, Brain Research Institute, Niigata University, Niigata, JapanDepartment of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, JapanDepartment of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, JapanDepartment of Comparative and Experimental Medicine, Brain Research Institute, Niigata University, Niigata, JapanDepartment of Animal Model Development, Brain Research Institute, Niigata University, Niigata, JapanDivision of Molecular and Diagnostic Pathology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, JapanDepartment of Medical Oncology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, JapanThe generation of mature, functional, thyroid follicular cells from pluripotent stem cells would potentially provide a therapeutic benefit for patients with hypothyroidism, but in vitro differentiation remains difficult. We earlier reported the in vivo generation of lung organs via blastocyst complementation in fibroblast growth factor 10 (Fgf10), compound, heterozygous mutant (Fgf10 Ex1mut/Ex3mut) mice. Fgf10 also plays an essential role in thyroid development and branching morphogenesis, but any role thereof in thyroid organogenesis remains unclear. Here, we report that the thyroids of Fgf10 Ex1mut/Ex3mut mice exhibit severe hypoplasia, and we generate thyroid tissues from mouse embryonic stem cells (ESCs) in Fgf10 Ex1mut/Ex3mut mice via blastocyst complementation. The tissues were morphologically normal and physiologically functional. The thyroid follicular cells of Fgf10 Ex1mut/Ex3mut chimeric mice were derived largely from GFP-positive mouse ESCs although the recipient cells were mixed. Thyroid generation in vivo via blastocyst complementation will aid functional thyroid regeneration.https://www.frontiersin.org/articles/10.3389/fendo.2020.609697/fullblastocyst complementationembryonic stem cellsFgf10pluripotent stem cellsthyroid generation
collection DOAJ
language English
format Article
sources DOAJ
author Qingsong Ran
Qiliang Zhou
Kanako Oda
Akihiro Yasue
Manabu Abe
Xulu Ye
Yingchun Li
Toshikuni Sasaoka
Kenji Sakimura
Yoichi Ajioka
Yasuo Saijo
spellingShingle Qingsong Ran
Qiliang Zhou
Kanako Oda
Akihiro Yasue
Manabu Abe
Xulu Ye
Yingchun Li
Toshikuni Sasaoka
Kenji Sakimura
Yoichi Ajioka
Yasuo Saijo
Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In Vivo
Frontiers in Endocrinology
blastocyst complementation
embryonic stem cells
Fgf10
pluripotent stem cells
thyroid generation
author_facet Qingsong Ran
Qiliang Zhou
Kanako Oda
Akihiro Yasue
Manabu Abe
Xulu Ye
Yingchun Li
Toshikuni Sasaoka
Kenji Sakimura
Yoichi Ajioka
Yasuo Saijo
author_sort Qingsong Ran
title Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In Vivo
title_short Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In Vivo
title_full Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In Vivo
title_fullStr Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In Vivo
title_full_unstemmed Generation of Thyroid Tissues From Embryonic Stem Cells via Blastocyst Complementation In Vivo
title_sort generation of thyroid tissues from embryonic stem cells via blastocyst complementation in vivo
publisher Frontiers Media S.A.
series Frontiers in Endocrinology
issn 1664-2392
publishDate 2020-12-01
description The generation of mature, functional, thyroid follicular cells from pluripotent stem cells would potentially provide a therapeutic benefit for patients with hypothyroidism, but in vitro differentiation remains difficult. We earlier reported the in vivo generation of lung organs via blastocyst complementation in fibroblast growth factor 10 (Fgf10), compound, heterozygous mutant (Fgf10 Ex1mut/Ex3mut) mice. Fgf10 also plays an essential role in thyroid development and branching morphogenesis, but any role thereof in thyroid organogenesis remains unclear. Here, we report that the thyroids of Fgf10 Ex1mut/Ex3mut mice exhibit severe hypoplasia, and we generate thyroid tissues from mouse embryonic stem cells (ESCs) in Fgf10 Ex1mut/Ex3mut mice via blastocyst complementation. The tissues were morphologically normal and physiologically functional. The thyroid follicular cells of Fgf10 Ex1mut/Ex3mut chimeric mice were derived largely from GFP-positive mouse ESCs although the recipient cells were mixed. Thyroid generation in vivo via blastocyst complementation will aid functional thyroid regeneration.
topic blastocyst complementation
embryonic stem cells
Fgf10
pluripotent stem cells
thyroid generation
url https://www.frontiersin.org/articles/10.3389/fendo.2020.609697/full
work_keys_str_mv AT qingsongran generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT qiliangzhou generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT kanakooda generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT akihiroyasue generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT manabuabe generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT xuluye generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT yingchunli generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT toshikunisasaoka generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT kenjisakimura generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT yoichiajioka generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
AT yasuosaijo generationofthyroidtissuesfromembryonicstemcellsviablastocystcomplementationinvivo
_version_ 1724383797959458816