Differential regulation of macrophage scavenger receptor isoforms: mRNA quantification using the polymerase chain reaction

There are two isoforms of the macrophage scavenger receptor (MSR I and II). Both are expressed on macrophages and mediate internalization of oxidized lipoproteins and several other ligands. MSR expression is regulated by cytokines but the individual regulation of each isoform is not well documented....

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Main Authors: M Dufva, A Svenningsson, G K Hansson
Format: Article
Language:English
Published: Elsevier 1995-11-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520397091
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spelling doaj-3e498372057b4eb5941fbfd22be07a8f2021-04-26T05:49:55ZengElsevierJournal of Lipid Research0022-22751995-11-01361122822290Differential regulation of macrophage scavenger receptor isoforms: mRNA quantification using the polymerase chain reactionM Dufva0A Svenningsson1G K Hansson2Department of Clinical Chemistry, Sahlgrenska University Hospital, University of Gothenburg, Sweden.Department of Clinical Chemistry, Sahlgrenska University Hospital, University of Gothenburg, Sweden.Department of Clinical Chemistry, Sahlgrenska University Hospital, University of Gothenburg, Sweden.There are two isoforms of the macrophage scavenger receptor (MSR I and II). Both are expressed on macrophages and mediate internalization of oxidized lipoproteins and several other ligands. MSR expression is regulated by cytokines but the individual regulation of each isoform is not well documented. We have therefore developed a PCR method to quantify mRNA levels of MSR isoforms. The analysis is based on relating the amount of reverse transcribed and amplified human macrophage MSR transcripts to a synthetic internal standard, using a 32P-labeled 5'-primer to allow quantitation of the products. Each MSR isoform and its corresponding standard amplified with equal efficiency and the amount of MSR mRNA could be determined from 1 to 100 ng of total RNA. Using this method, we estimated that each monocyte-derived macrophage contains 10-130 molecules of MSR I and 30-640 copies of MSR II mRNA. Both isoforms were down-regulated by bacterial endotoxin (LPS), but the effect was more pronounced for MSR II transcripts. However, cycloheximide induced a selective degradation of MSR I transcripts, leaving MSR II levels unaltered. This suggests that both transcriptional and posttranscriptional control mechanisms are important in the regulation of MSR expression.http://www.sciencedirect.com/science/article/pii/S0022227520397091
collection DOAJ
language English
format Article
sources DOAJ
author M Dufva
A Svenningsson
G K Hansson
spellingShingle M Dufva
A Svenningsson
G K Hansson
Differential regulation of macrophage scavenger receptor isoforms: mRNA quantification using the polymerase chain reaction
Journal of Lipid Research
author_facet M Dufva
A Svenningsson
G K Hansson
author_sort M Dufva
title Differential regulation of macrophage scavenger receptor isoforms: mRNA quantification using the polymerase chain reaction
title_short Differential regulation of macrophage scavenger receptor isoforms: mRNA quantification using the polymerase chain reaction
title_full Differential regulation of macrophage scavenger receptor isoforms: mRNA quantification using the polymerase chain reaction
title_fullStr Differential regulation of macrophage scavenger receptor isoforms: mRNA quantification using the polymerase chain reaction
title_full_unstemmed Differential regulation of macrophage scavenger receptor isoforms: mRNA quantification using the polymerase chain reaction
title_sort differential regulation of macrophage scavenger receptor isoforms: mrna quantification using the polymerase chain reaction
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1995-11-01
description There are two isoforms of the macrophage scavenger receptor (MSR I and II). Both are expressed on macrophages and mediate internalization of oxidized lipoproteins and several other ligands. MSR expression is regulated by cytokines but the individual regulation of each isoform is not well documented. We have therefore developed a PCR method to quantify mRNA levels of MSR isoforms. The analysis is based on relating the amount of reverse transcribed and amplified human macrophage MSR transcripts to a synthetic internal standard, using a 32P-labeled 5'-primer to allow quantitation of the products. Each MSR isoform and its corresponding standard amplified with equal efficiency and the amount of MSR mRNA could be determined from 1 to 100 ng of total RNA. Using this method, we estimated that each monocyte-derived macrophage contains 10-130 molecules of MSR I and 30-640 copies of MSR II mRNA. Both isoforms were down-regulated by bacterial endotoxin (LPS), but the effect was more pronounced for MSR II transcripts. However, cycloheximide induced a selective degradation of MSR I transcripts, leaving MSR II levels unaltered. This suggests that both transcriptional and posttranscriptional control mechanisms are important in the regulation of MSR expression.
url http://www.sciencedirect.com/science/article/pii/S0022227520397091
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