Karakterisasi protease Bacillus sp. UGM5
The objective of this experiment is to indentify the characters of proetease produced by Bacillus sp.UGM5.the protease secreted by Bacillus sp.UGM5 was first isolated,purified and then charactirezed.The crude enzyme has spesific actifity of 1.14 U/mg,however,the spesific activity of purified enzyme...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Center for Leather, Rubber, and Plastics
1999-07-01
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| Series: | Majalah Kulit, Karet, dan Plastik |
| Online Access: | http://ejournal.kemenperin.go.id/mkkp/article/view/297/274 |
| Summary: | The objective of this experiment is to indentify the characters of proetease produced by Bacillus sp.UGM5.the protease secreted by Bacillus sp.UGM5 was first isolated,purified and then charactirezed.The crude enzyme has spesific actifity of 1.14 U/mg,however,the spesific activity of purified enzyme was increased by 23.8 times fold and recovery was 33.69%.The Page of nondenatured crude enzymes showes two type of proreases,however ,the SDS-Page of denatured purified enzyme showed four protein-bends with molecular weights of 55.5 kDa,18kDa respecetively.The optimum pH and temperature for the enzyme acrivity are 8.5 and 420C and belongs to serin protease type,with Km 3 X 10-3mM and Vmax 0.0890mM/30 minutes.The activity is not inhibited by Ca+2,Fe+2 and EDTA. |
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| ISSN: | 1829-6971 2460-4461 |