Sugar microarray via click chemistry: molecular recognition with lectins and amyloid β (1–42)

• Main Author: Erino Matsumoto, Takahiro Yamauchi, Tomohiro Fukuda and Yoshiko Miura
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2009-01-01
• Series: Science and Technology of Advanced Materials
• Subjects: self-assembled monolayer; saccharide; microarray; surface plasmon resonance; amyloid; Alzheimer
• Online Access: http://www.iop.org/EJ/abstract/1468-6996/10/3/034605

Sugar microarrays were fabricated on various substrates via click chemistry. Acetylene-terminated substrates were prepared by forming self-assembled monolayers (SAMs) on a gold substrate with alkyl-disulfide and on silicon, quartz and glass substrates with a silane-coupling reagent. The gold substrates were subjected to surface plasmon resonance measurements, and the quartz and glass substrates were subjected to spectroscopy measurements and optical microscopy observation. The saccharide-immobilized substrate on the gold substrate showed specific interaction with the corresponding lectin, and the saccharides showed inert surface properties to other proteins with a high signal-to-noise ratio. We also focused on the saccharide–protein interaction on protein amyloidosis of Alzheimer amyloid β. Amyloid β peptide showed conformation transition on the saccharide-immobilization substrate into a β-sheet, and fibril formation and amyloid aggregates were found on the specific saccharides.