A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here w...

Full description

Bibliographic Details
Main Authors: Alisha House, Erica Fatica, Rohan Shah, Jared Stergar, Ryan Pearce, Yana Sandlers
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:MethodsX
Subjects:
Induced pluripotent stem cell-derived cardiomyocytes
Metabolic characterization
GCMS
LC–MS/MS
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016119301487
id doaj-3e3061098ff944d193554e8f5ab991c0
record_format Article
spelling doaj-3e3061098ff944d193554e8f5ab991c02021-01-02T05:09:52ZengElsevierMethodsX2215-01612020-01-017100572A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)Alisha House0Erica Fatica1Rohan Shah2Jared Stergar3Ryan Pearce4Yana Sandlers5Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United StatesDepartment of Chemistry, Cleveland State University, Cleveland, OH 44115, United StatesDepartment of Chemistry, Cleveland State University, Cleveland, OH 44115, United StatesDepartment of Chemistry, Cleveland State University, Cleveland, OH 44115, United StatesDepartment of Chemistry, Cleveland State University, Cleveland, OH 44115, United StatesCorresponding author.; Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United StatesRecent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.http://www.sciencedirect.com/science/article/pii/S2215016119301487Induced pluripotent stem cell-derived cardiomyocytesMetabolic characterizationGCMSLC–MS/MS
collection DOAJ
language English
format Article
sources DOAJ
author Alisha House
Erica Fatica
Rohan Shah
Jared Stergar
Ryan Pearce
Yana Sandlers
spellingShingle Alisha House
Erica Fatica
Rohan Shah
Jared Stergar
Ryan Pearce
Yana Sandlers
A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
MethodsX
Induced pluripotent stem cell-derived cardiomyocytes
Metabolic characterization
GCMS
LC–MS/MS
author_facet Alisha House
Erica Fatica
Rohan Shah
Jared Stergar
Ryan Pearce
Yana Sandlers
author_sort Alisha House
title A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_short A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_full A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_fullStr A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_full_unstemmed A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
title_sort protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (ips-cm)
publisher Elsevier
series MethodsX
issn 2215-0161
publishDate 2020-01-01
description Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.
topic Induced pluripotent stem cell-derived cardiomyocytes
Metabolic characterization
GCMS
LC–MS/MS
url http://www.sciencedirect.com/science/article/pii/S2215016119301487
work_keys_str_mv AT alishahouse aprotocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT ericafatica aprotocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT rohanshah aprotocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT jaredstergar aprotocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT ryanpearce aprotocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT yanasandlers aprotocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT alishahouse protocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT ericafatica protocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT rohanshah protocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT jaredstergar protocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT ryanpearce protocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
AT yanasandlers protocolformetaboliccharacterizationofhumaninducedpluripotentstemcellderivedcardiomyocytesipscm
_version_ 1724359496118042624

Similar Items