A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)

Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here w...

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Bibliographic Details
Main Authors: Alisha House, Erica Fatica, Rohan Shah, Jared Stergar, Ryan Pearce, Yana Sandlers
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:MethodsX
Subjects:
Induced pluripotent stem cell-derived cardiomyocytes
Metabolic characterization
GCMS
LC–MS/MS
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016119301487
Description
Summary:Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.
ISSN:2215-0161

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