Detection of Escherichia coli hlyA gene and Staphylococcus aureus Sea gene in raw milk of buffaloes using RT-PCR technique in AL- Qadisiyah province

• Main Authors: J.N. Sadeq, Kh.H. Fahed, H.J. Hassan
• Format: Article
• Language: Arabic
• Published: University of Mosul, College of Veterinary Medicine 2018-06-01
• Series: Iraqi Journal of Veterinary Sciences
• Subjects: real; time polymerase chain reaction; escherichia coli; hlya gene; staphylococcus aureus; sea gene
• Online Access: https://vetmedmosul.com/article_153815_69457736e32016f7588068e9d7427537.pdf

The aim of this study was to determines the prevalence of virulence gene hemolysin A (hly A) <em>Escherichia coli</em> and Staphylococcal enterotoxins (sea) in <em>Staphylococcus</em> <em>aureus</em> in raw milk buffaloes. In molecular laboratory, real-time polymerase chain reaction (RT-PCR) technique has been performed for 24 samples which have been taken randomly from Buffaloes milk, using primers of high specificity for <em>Escherichia</em> <em>coli</em> hlyA gene and <em>Staphylococcus</em> <em>aureus</em> Sea genes. The results showed different degrees of the studied genes activities. Four out of 24 samples represented <em>S. aureus</em> Sea gene (16.6%) whereas 16 out of 24 samples represented <em>E. coli</em> hlyA gene (66.6%). this study concluded that buffaloes milk might be a source of contamination with pathogenic bacteria of virulent genes which may have different levels of activities.